Detailed information of TA system TA158404

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 1537686..1538332
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: FOC65_RS06915
• Protein ID: WP_003403137.1
• Coordinates: 1537686..1538099 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: FOC65_RS06920
• Protein ID: WP_003403139.1
• Coordinates: 1538096..1538332 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS06895	1533769..1535319	+	1551	WP_003403119.1	MCE family protein	-
FOC65_RS06900	1535371..1535763	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
FOC65_RS06905	1535760..1536017	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
FOC65_RS06910	1536200..1537435	-	1236	WP_003403128.1	ATP-binding protein	-
FOC65_RS06915	1537686..1538099	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC65_RS06920	1538096..1538332	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOC65_RS06925	1538436..1538942	-	507	WP_003900973.1	two component system sensor kinase HK1	-
FOC65_RS06930	1539056..1539526	-	471	WP_003403144.1	HAMP domain-containing protein	-
FOC65_RS06935	1539570..1540331	-	762	WP_003403148.1	two-component system response regulator TcrA	-
FOC65_RS06940	1540388..1540699	+	312	WP_003403164.1	hypothetical protein	-
FOC65_RS06945	1540771..1541721	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
FOC65_RS06950	1541962..1542546	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
FOC65_RS06955	1542548..1543258	+	711	Protein_1377	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T158404 WP_003403137.1 NZ_CP054013:c1538099-1537686 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T158404 NZ_CP069633:3172060-3172233 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT158404 WP_003403139.1 NZ_CP054013:c1538332-1538096 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT158404 NZ_CP069633:3171736-3172032 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89