Detailed information of TA system TA158396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1173237..1173871
• Replicon: chromosome
• Accession: NZ_CP054013
• Organism: Mycobacterium tuberculosis strain FDAARGOS_757

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF84
• Locus tag: FOC65_RS05265
• Protein ID: WP_003401406.1
• Coordinates: 1173237..1173665 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: FOC65_RS05270
• Protein ID: WP_011961607.1
• Coordinates: 1173689..1173871 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC65_RS05240	1169104..1170237	-	1134	WP_003401394.1	hypothetical protein	-
FOC65_RS05245	1170234..1170854	-	621	WP_003401397.1	TetR/AcrR family transcriptional regulator	-
FOC65_RS05250	1170951..1171532	+	582	WP_003401399.1	VOC family protein	-
FOC65_RS05255	1171462..1172187	-	726	WP_003899886.1	TetR/AcrR family transcriptional regulator	-
FOC65_RS05260	1172277..1173197	+	921	WP_003401404.1	DUF2236 domain-containing protein	-
FOC65_RS05265	1173237..1173665	-	429	WP_003401406.1	type II toxin-antitoxin system VapC family toxin	Toxin
FOC65_RS05270	1173689..1173871	-	183	WP_011961607.1	hypothetical protein	Antitoxin
FOC65_RS05275	1173966..1176839	-	2874	WP_011961608.1	PE family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15887.27 Da
• Isoelectric Point: 7.2214

>T158396 WP_003401406.1 NZ_CP054013:c1173665-1173237 [Mycobacterium tuberculosis]
MFLIDVNVLLAAHRGDHPNHRTVRPWFDRLLAADDPFTVPNLVWASFLRLTTNRRIFEIPSPRADAFAFVEAVNAQPHHL
PTSPGPRHLVLLRKLCDEADASGDLIPDAVLGAIAVEHHCAVVSLDRDFARFASVRHIRPPI

Nucleotide

• Length: 429 bp

>T158396 NZ_CP069633:c739442-739188 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6262.06 Da
• Isoelectric Point: 4.5878

>AT158396 WP_011961607.1 NZ_CP054013:c1173871-1173689 [Mycobacterium tuberculosis]
MGAVIEDALRRELAAARTGGARPTVPVFDAGTGPRPGIDLTSNTVLSEVLDEGLELNSRK

Nucleotide

• Length: 183 bp

>AT158396 NZ_CP069633:c739690-739439 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A7U4BRM6