158385

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2911816..2912458 Replicon chromosome
Accession NZ_CP053997
Organism Bacillus cereus strain FDAARGOS_780

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC74_RS16830 Protein ID WP_000635963.1
Coordinates 2911816..2912166 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC74_RS16835 Protein ID WP_000004570.1
Coordinates 2912171..2912458 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC74_RS16815 2908752..2909210 - 459 WP_000344248.1 SprT family protein -
FOC74_RS16820 2909406..2909522 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC74_RS16825 2909580..2911748 - 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC74_RS16830 2911816..2912166 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC74_RS16835 2912171..2912458 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC74_RS16840 2912768..2913937 - 1170 WP_000390600.1 alanine racemase -
FOC74_RS16845 2914056..2915006 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC74_RS16850 2915163..2915522 - 360 WP_000635040.1 holo-ACP synthase -
FOC74_RS16855 2915615..2916187 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC74_RS16860 2916180..2917142 - 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158385 WP_000635963.1 NZ_CP053997:c2912166-2911816 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158385 NZ_CP069630:c3510895-3510743 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158385 WP_000004570.1 NZ_CP053997:c2912458-2912171 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158385 NZ_CP069630:3510944-3511001 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References