158383

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 5036510..5037152 Replicon chromosome
Accession NZ_CP053991
Organism Bacillus cereus strain FDAARGOS_781

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC75_RS26615 Protein ID WP_000635963.1
Coordinates 5036802..5037152 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC75_RS26610 Protein ID WP_000004570.1
Coordinates 5036510..5036797 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC75_RS26585 5031826..5032788 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
FOC75_RS26590 5032781..5033353 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC75_RS26595 5033446..5033805 + 360 WP_000635040.1 holo-ACP synthase -
FOC75_RS26600 5033962..5034912 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC75_RS26605 5035031..5036200 + 1170 WP_000390600.1 alanine racemase -
FOC75_RS26610 5036510..5036797 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC75_RS26615 5036802..5037152 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC75_RS26620 5037220..5039388 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC75_RS26625 5039446..5039562 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC75_RS26630 5039758..5040216 + 459 WP_000344248.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158383 WP_000635963.1 NZ_CP053991:5036802-5037152 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158383 NZ_CP069630:3360417-3360794 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158383 WP_000004570.1 NZ_CP053991:5036510-5036797 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158383 NZ_CP069630:3359959-3360327 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References