Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 542107..542743 Replicon chromosome
Accession NZ_CP053989
Organism Bacillus circulans strain FDAARGOS_783

Toxin (Protein)


Gene name mazF Uniprot ID R9C9X9
Locus tag FOC77_RS02775 Protein ID WP_016201246.1
Coordinates 542393..542743 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A0J1I9K1
Locus tag FOC77_RS02770 Protein ID WP_047944180.1
Coordinates 542107..542388 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC77_RS02750 537947..538672 - 726 WP_047944184.1 rhomboid family intramembrane serine protease -
FOC77_RS02755 538782..539132 + 351 WP_047944183.1 holo-ACP synthase -
FOC77_RS02760 539450..540460 + 1011 WP_047944182.1 outer membrane lipoprotein carrier protein LolA -
FOC77_RS02765 540614..541771 + 1158 WP_047944181.1 alanine racemase -
FOC77_RS02770 542107..542388 + 282 WP_047944180.1 CopG family ribbon-helix-helix protein Antitoxin
FOC77_RS02775 542393..542743 + 351 WP_016201246.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC77_RS02780 542866..543075 + 210 WP_047944179.1 hypothetical protein -
FOC77_RS02785 543272..543637 + 366 WP_047944178.1 STAS domain-containing protein -
FOC77_RS02790 543641..544042 + 402 WP_047944177.1 anti-sigma regulatory factor -
FOC77_RS02795 544054..545064 + 1011 WP_047944176.1 PP2C family protein-serine/threonine phosphatase -
FOC77_RS02800 545120..545452 + 333 WP_047944175.1 anti-sigma factor antagonist -
FOC77_RS02805 545449..545919 + 471 WP_047944173.1 anti-sigma B factor RsbW -
FOC77_RS02810 545897..546694 + 798 WP_047944172.1 RNA polymerase sigma factor SigB -
FOC77_RS02815 546691..547287 + 597 WP_047944171.1 protein phosphatase 2C domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13048.11 Da        Isoelectric Point: 4.9105

>T158381 WP_016201246.1 NZ_CP053989:542393-542743 [Bacillus circulans]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIIAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDEEMMEKVDEALQISLGLIEF

Download         Length: 351 bp

>T158381 NZ_CP069630:2864232-2864543 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10869.46 Da        Isoelectric Point: 8.4775

>AT158381 WP_047944180.1 NZ_CP053989:542107-542388 [Bacillus circulans]
VSESSTTTEIMIKLPKHLLTELDGFVKQENVNRSEFIYQATKMYLRERKKRHIRESMRRGYMEMAKLNLSIASESFLAEY
EAEHTVERLVSGG

Download         Length: 282 bp

>AT158381 NZ_CP069630:2864544-2864834 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB R9C9X9

Antitoxin

Source ID Structure
AlphaFold DB A0A0J1I9K1

References