Detailed information of TA system TA158380

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09907-MazE
• Location: 1489888..1490486
• Replicon: chromosome
• Accession: NZ_CP053988
• Organism: Abiotrophia defectiva strain FDAARGOS_785

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: W1Q348
• Locus tag: FOC79_RS06855
• Protein ID: WP_023391686.1
• Coordinates: 1489888..1490226 (-)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: W1Q360
• Locus tag: FOC79_RS06860
• Protein ID: WP_023391687.1
• Coordinates: 1490220..1490486 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC79_RS06815	1485452..1485650	+	199	Protein_1301	IS3 family transposase	-
FOC79_RS06820	1485960..1486475	-	516	WP_035364127.1	DUF308 domain-containing protein	-
FOC79_RS06825	1486840..1487010	+	171	WP_023391680.1	hypothetical protein	-
FOC79_RS06830	1487191..1487391	-	201	Protein_1304	XRE family transcriptional regulator	-
FOC79_RS06835	1487634..1488671	+	1038	WP_023391682.1	M42 family metallopeptidase	-
FOC79_RS06840	1488699..1489148	+	450	WP_035364587.1	GNAT family N-acetyltransferase	-
FOC79_RS06845	1489255..1489512	+	258	WP_023391684.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
FOC79_RS06850	1489502..1489831	+	330	WP_023391685.1	type II toxin-antitoxin system RelE/ParE family toxin	-
FOC79_RS06855	1489888..1490226	-	339	WP_023391686.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
FOC79_RS06860	1490220..1490486	-	267	WP_023391687.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
FOC79_RS06865	1490782..1492287	+	1506	WP_035364129.1	PTS transporter subunit EIIC	-
FOC79_RS06870	1492416..1493372	+	957	WP_023391689.1	alpha/beta hydrolase	-
FOC79_RS06875	1493416..1493625	+	210	WP_023391690.1	hypothetical protein	-
FOC79_RS06880	1493781..1495184	+	1404	WP_023391691.1	dNTP triphosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-106)

Antitoxin

• SpoVT-AbrB domain (13-53)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12760.76 Da
• Isoelectric Point: 8.5217

>T158380 WP_023391686.1 NZ_CP053988:c1490226-1489888 [Abiotrophia defectiva]
MVIKQGDIFYVDFNPSKGHEQKHRRPALALSHSLLTKFSGLTIVAPITTTSRNFPTYHELTSTRTIQGKVMLDQTIALDL
AARGVTKVEERLSQEELKLILDKYKLLFDMED

Nucleotide

• Length: 339 bp

>T158380 NZ_CP053988:c1490226-1489888 [Abiotrophia defectiva]
ATGGTAATCAAACAAGGCGATATTTTCTACGTTGACTTCAATCCCAGCAAGGGCCACGAGCAGAAGCATCGTCGCCCGGC
TCTCGCTCTAAGCCATAGCCTCCTGACTAAGTTCAGCGGCTTAACCATCGTGGCCCCCATCACCACCACCAGTCGCAACT
TTCCCACCTATCATGAGCTGACTAGCACTCGTACCATCCAAGGCAAGGTCATGCTAGACCAGACCATCGCCCTAGACTTG
GCGGCACGAGGTGTTACTAAGGTAGAGGAAAGACTATCCCAAGAAGAATTGAAGCTTATCTTGGATAAGTATAAGTTGCT
GTTTGATATGGAGGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9954.07 Da
• Isoelectric Point: 4.3085

>AT158380 WP_023391687.1 NZ_CP053988:c1490486-1490220 [Abiotrophia defectiva]
MESQAQYRTTLSQWGNSKAVRLPAAILKKLNLLDNQTLLVSVEDNSIRLSPEQKQASTIHELFAEWDQDGLGEGELDWGQ
AKGQELEW

Nucleotide

• Length: 267 bp

>AT158380 NZ_CP053988:c1490486-1490220 [Abiotrophia defectiva]
ATGGAGTCACAAGCACAATACCGGACCACACTATCCCAATGGGGCAATTCTAAGGCCGTGCGCCTACCTGCTGCCATTCT
CAAAAAGTTAAATCTATTGGACAATCAAACCCTTCTAGTCTCTGTCGAGGATAACAGCATTCGCCTAAGTCCTGAGCAAA
AGCAAGCTTCCACCATCCATGAGTTGTTTGCTGAATGGGATCAAGACGGCCTTGGAGAAGGCGAACTTGATTGGGGTCAG
GCGAAAGGTCAGGAACTAGAATGGTAA

Structures

Toxin

• AlphaFold DB: W1Q348

Antitoxin

• AlphaFold DB: W1Q360