Detailed information of TA system TA158379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 5937058..5938724
• Replicon: chromosome
• Accession: NZ_CP053987
• Organism: Achromobacter denitrificans strain FDAARGOS_786

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: FOC80_RS27630
• Protein ID: WP_062683046.1
• Coordinates: 5937058..5937897 (+)
• Length: 280 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: FOC80_RS27635
• Protein ID: WP_062683047.1
• Coordinates: 5937909..5938724 (+)
• Length: 272 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC80_RS27605	5932577..5933335	-	759	WP_075873249.1	ABC transporter permease	-
FOC80_RS27610	5933337..5934125	-	789	WP_075873250.1	ABC transporter ATP-binding protein	-
FOC80_RS27615	5934118..5935119	-	1002	WP_062683044.1	ABC transporter substrate-binding protein	-
FOC80_RS27620	5935165..5935755	-	591	WP_062683070.1	cupin domain-containing protein	-
FOC80_RS27625	5935850..5936905	-	1056	WP_062683045.1	porin	-
FOC80_RS27630	5937058..5937897	+	840	WP_062683046.1	alpha/beta hydrolase	Toxin
FOC80_RS27635	5937909..5938724	+	816	WP_062683047.1	IclR family transcriptional regulator	Antitoxin
FOC80_RS27640	5938786..5939577	+	792	WP_062683048.1	SDR family oxidoreductase	-
FOC80_RS27645	5939570..5941240	+	1671	WP_062683049.1	thiamine pyrophosphate-binding protein	-
FOC80_RS27650	5941248..5942045	+	798	WP_062683050.1	aspartate dehydrogenase	-
FOC80_RS27655	5942053..5943540	+	1488	WP_062683051.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (39-270)

Antitoxin

• Transcription regulator IclR, C-terminal (138-260)

Sequences

Toxin

Protein

• Length: 280 a.a.
• Molecular weight: 29151.29 Da
• Isoelectric Point: 7.8874

>T158379 WP_062683046.1 NZ_CP053987:5937058-5937897 [Achromobacter denitrificans]
MPATEQAMRDQALHRFTACLAPTAQGPSAYRRLGTGPAVVLLHGIGSGSGCWLDAATALAGECTVLAWDAPGYGASAPLA
GDRPSAADYAERLHQVLRHAGIDECVLVGHSLGALVAAAYCARRERARISRLVLLSPARGYGSADAGEREATRGKRLGAL
ERLGIDGMAAARASALLSPAAQAGQVQWAAWNMRQITPEGYRQAVGLLCDGDLLAFPVPPMSVDVRVGIHDTITPAPKCQ
AIAAAWRASFELIPGAGHACHIERPQEVAEIVRQARAGS

Nucleotide

• Length: 840 bp

>T158379 NZ_CP069630:2022971-2023435 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGCGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAGCCATTGA

Antitoxin

Protein

• Length: 272 a.a.
• Molecular weight: 29497.47 Da
• Isoelectric Point: 6.0246

>AT158379 WP_062683047.1 NZ_CP053987:5937909-5938724 [Achromobacter denitrificans]
MSDTDVNDDGRDRYTVPALERGLRLLCQFNRNDRALGAPELARRLDLPRSTVFRMLNTLEHMGFVRKTDGGSTYQLDMGV
LRLGFEYLASLELTELGRPLLERVRDATGYPSNLVVLDQHWAVVVAKSSAPTPFVSTVYVGTRLPAHATLLGRALLCDLS
LDALRNLYGDAPLQSYSPRTPGTVEALHEVLQQDRARGYVSDHGFYEAAVASIAAPVRDRNGATVAALGVTIPTMTTTQP
AMDEAGVIATVRSAADELSRLLDYAPPGATR

Nucleotide

• Length: 816 bp

>AT158379 NZ_CP069630:2022636-2022971 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCTGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA