Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1125917..1126559 Replicon chromosome
Accession NZ_CP053981
Organism Bacillus thuringiensis strain FDAARGOS_793

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC87_RS05960 Protein ID WP_000635963.1
Coordinates 1126209..1126559 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC87_RS05955 Protein ID WP_000004570.1
Coordinates 1125917..1126204 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC87_RS05930 1121233..1122195 + 963 WP_000961158.1 UV DNA damage repair endonuclease UvsE -
FOC87_RS05935 1122188..1122760 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC87_RS05940 1122853..1123212 + 360 WP_000635040.1 holo-ACP synthase -
FOC87_RS05945 1123369..1124319 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC87_RS05950 1124438..1125607 + 1170 WP_000390600.1 alanine racemase -
FOC87_RS05955 1125917..1126204 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC87_RS05960 1126209..1126559 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC87_RS05965 1126627..1128795 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC87_RS05970 1128853..1128969 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC87_RS05975 1129165..1129623 + 459 WP_000344244.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158369 WP_000635963.1 NZ_CP053981:1126209-1126559 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158369 NZ_CP069628:1791-2075 [Escherichia coli]
ATGAGCTATAACCTTGAATTTGATCCCCGAGCCCTGAAGGAATGGCGCAAGCTCGGGGATGATGTCCGTCTGCAGTTCAA
GAAAAAACTCGAGCAGGTTCTACAACACCCGCGGATCGATAAAAATCGCCTGCGAGAGCTGCATGACTGCTACAAAATCA
AGCTCCGTGCATCCGGTTATCGCTTGGTCTATCAGGTTCGCGATCAAACCATTACGGTATTCGTGGTGGCGGTCGGTAAG
CGCGAGCGTTCTGCCGCTTACGATGCGGCCGATAAACGCTTATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158369 WP_000004570.1 NZ_CP053981:1125917-1126204 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158369 NZ_CP069628:1559-1801 [Escherichia coli]
ATGGCCACGATTAATATTCGGATCGATGACGAGCTGAAAAGCCGCTCTTATGCCGCACTGGAAAAGCTGGGCGTAACGCC
GTCCGAGGTTCTGCGCCAAACACTGGAATATGTGGCCCAAAGCGGACGTTTGCCGTTCCAGCAGGTTTTGCTGACCGAGG
ATGATGCCGATTTGATGGCTATCGTTCGGGATCGTCTGGAAAACCCACAGGCGGGCGTAAAGGTGTCACTGGATGAGCTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References