Detailed information of TA system TA158366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 4719642..4720620
• Replicon: chromosome
• Accession: NZ_CP053972
• Organism: Bacillus thuringiensis strain FDAARGOS_796

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: FOC90_RS27945
• Protein ID: WP_000624969.1
• Coordinates: 4719642..4720379 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: B7IX39
• Locus tag: FOC90_RS27950
• Protein ID: WP_000237817.1
• Coordinates: 4720492..4720620 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FOC90_RS27915	4715233..4715640	+	408	WP_000072495.1	VOC family protein	-
FOC90_RS27920	4715985..4716767	+	783	WP_000381785.1	class I SAM-dependent methyltransferase	-
FOC90_RS27925	4716921..4717766	-	846	Protein_4699	alpha-keto acid decarboxylase family protein	-
FOC90_RS27930	4717742..4717870	-	129	WP_003265031.1	hypothetical protein	-
FOC90_RS27935	4718018..4718500	+	483	WP_000191905.1	MarR family winged helix-turn-helix transcriptional regulator	-
FOC90_RS27940	4718666..4719403	+	738	WP_000594159.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
FOC90_RS27945	4719642..4720379	+	738	WP_000624969.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
FOC90_RS27950	4720492..4720620	+	129	WP_000237817.1	hypothetical protein	Antitoxin
FOC90_RS27955	4720693..4720869	+	177	WP_000852629.1	stage II sporulation protein SB	-
FOC90_RS27960	4720888..4721277	-	390	WP_000712655.1	YxeA family protein	-
FOC90_RS27965	4721489..4722958	+	1470	WP_000287541.1	beta-Ala-His dipeptidase	-
FOC90_RS27970	4723271..4724080	+	810	WP_000678376.1	C39 family peptidase	-
FOC90_RS27975	4724106..4724711	+	606	WP_000527414.1	hypothetical protein	-
FOC90_RS27980	4724852..4725103	-	252	WP_000821942.1	response regulator transcription factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (11-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28383.13 Da
• Isoelectric Point: 8.2998

>T158366 WP_000624969.1 NZ_CP053972:4719642-4720379 [Bacillus thuringiensis]
MISNIRIGLFVLAIFFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNVFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T158366 NZ_CP069627:c4039654-4039436 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4981.37 Da
• Isoelectric Point: 3.9150

>AT158366 WP_000237817.1 NZ_CP053972:4720492-4720620 [Bacillus thuringiensis]
VVKDYDNDFFKEDNEDTTDFSLIKGDALQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT158366 NZ_CP069627:c4040054-4039680 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: B7IX39