Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2643968..2644610 Replicon chromosome
Accession NZ_CP053972
Organism Bacillus thuringiensis strain FDAARGOS_796

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FOC90_RS17365 Protein ID WP_000635965.1
Coordinates 2644260..2644610 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC90_RS17360 Protein ID WP_000004570.1
Coordinates 2643968..2644255 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC90_RS17335 2639285..2640247 + 963 WP_000961146.1 UV DNA damage repair endonuclease UvsE -
FOC90_RS17340 2640240..2640812 - 573 WP_000908528.1 rhomboid family intramembrane serine protease -
FOC90_RS17345 2640906..2641265 + 360 WP_000583420.1 holo-ACP synthase -
FOC90_RS17350 2641422..2642372 + 951 WP_003309006.1 outer membrane lipoprotein carrier protein LolA -
FOC90_RS17355 2642490..2643659 + 1170 WP_000390594.1 alanine racemase -
FOC90_RS17360 2643968..2644255 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC90_RS17365 2644260..2644610 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC90_RS17370 2644678..2646846 + 2169 WP_000426211.1 RNA-binding transcriptional accessory protein -
FOC90_RS17375 2646904..2647020 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
FOC90_RS17380 2647216..2647674 + 459 WP_000343552.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T158365 WP_000635965.1 NZ_CP053972:2644260-2644610 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T158365 NZ_CP069627:c3746968-3746591 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158365 WP_000004570.1 NZ_CP053972:2643968-2644255 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158365 NZ_CP069627:c3747389-3747015 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References