Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 24912..25554 Replicon chromosome
Accession NZ_CP053965
Organism Bacillus cereus strain FDAARGOS_802

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC96_RS03560 Protein ID WP_000635963.1
Coordinates 24912..25262 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC96_RS03565 Protein ID WP_000004570.1
Coordinates 25267..25554 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC96_RS03545 21848..22306 - 459 WP_000344244.1 SprT family protein -
FOC96_RS03550 22502..22618 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC96_RS03555 22676..24844 - 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC96_RS03560 24912..25262 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC96_RS03565 25267..25554 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC96_RS03570 25864..27033 - 1170 WP_000390600.1 alanine racemase -
FOC96_RS03575 27152..28102 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC96_RS03580 28259..28618 - 360 WP_000635040.1 holo-ACP synthase -
FOC96_RS03585 28711..29283 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC96_RS03590 29276..30238 - 963 WP_000961158.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158363 WP_000635963.1 NZ_CP053965:c25262-24912 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158363 NZ_CP069627:c3510894-3510742 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158363 WP_000004570.1 NZ_CP053965:c25554-25267 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158363 NZ_CP069627:3510943-3511000 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References