158350

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 720771..721413 Replicon chromosome
Accession NZ_CP053955
Organism Bacillus tropicus strain FDAARGOS_782

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC76_RS03525 Protein ID WP_000635963.1
Coordinates 720771..721121 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC76_RS03530 Protein ID WP_000004570.1
Coordinates 721126..721413 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC76_RS03510 717707..718165 - 459 WP_000344256.1 SprT family protein -
FOC76_RS03515 718361..718477 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC76_RS03520 718535..720703 - 2169 WP_000426233.1 RNA-binding transcriptional accessory protein -
FOC76_RS03525 720771..721121 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC76_RS03530 721126..721413 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC76_RS03535 721723..722892 - 1170 WP_000390607.1 alanine racemase -
FOC76_RS03540 723011..723961 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC76_RS03545 724118..724477 - 360 WP_000635043.1 holo-ACP synthase -
FOC76_RS03550 724570..725142 + 573 WP_000906921.1 rhomboid family intramembrane serine protease -
FOC76_RS03555 725135..726097 - 963 WP_000961159.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158350 WP_000635963.1 NZ_CP053955:c721121-720771 [Bacillus tropicus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158350 NZ_CP069627:c398025-397923 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158350 WP_000004570.1 NZ_CP053955:c721413-721126 [Bacillus tropicus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158350 NZ_CP069627:397885-398153 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References