Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2670316..2670958 Replicon chromosome
Accession NZ_CP053954
Organism Bacillus cereus strain FDAARGOS_798

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FOC92_RS17100 Protein ID WP_000635965.1
Coordinates 2670608..2670958 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC92_RS17095 Protein ID WP_000004570.1
Coordinates 2670316..2670603 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC92_RS17070 2665633..2666595 + 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -
FOC92_RS17075 2666588..2667160 - 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FOC92_RS17080 2667254..2667613 + 360 WP_000583417.1 holo-ACP synthase -
FOC92_RS17085 2667770..2668720 + 951 WP_002060618.1 outer membrane lipoprotein carrier protein LolA -
FOC92_RS17090 2668838..2670007 + 1170 WP_000390617.1 alanine racemase -
FOC92_RS17095 2670316..2670603 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC92_RS17100 2670608..2670958 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC92_RS17105 2671026..2673194 + 2169 WP_172852688.1 RNA-binding transcriptional accessory protein -
FOC92_RS17110 2673252..2673368 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC92_RS17115 2673564..2674022 + 459 WP_000344240.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T158348 WP_000635965.1 NZ_CP053954:2670608-2670958 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T158348 NZ_CP069627:c68761-68483 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158348 WP_000004570.1 NZ_CP053954:2670316-2670603 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158348 NZ_CP069627:c68483-68199 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References