Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 3386958..3387600 Replicon chromosome
Accession NZ_CP053951
Organism Bacillus cereus strain FDAARGOS_799

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag FOC93_RS20400 Protein ID WP_000635965.1
Coordinates 3386958..3387308 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC93_RS20405 Protein ID WP_000004570.1
Coordinates 3387313..3387600 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC93_RS20385 3383894..3384352 - 459 WP_000344240.1 SprT family protein -
FOC93_RS20390 3384548..3384664 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC93_RS20395 3384722..3386890 - 2169 WP_172852688.1 RNA-binding transcriptional accessory protein -
FOC93_RS20400 3386958..3387308 - 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC93_RS20405 3387313..3387600 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC93_RS20410 3387909..3389078 - 1170 WP_000390617.1 alanine racemase -
FOC93_RS20415 3389196..3390146 - 951 WP_002060618.1 outer membrane lipoprotein carrier protein LolA -
FOC93_RS20420 3390303..3390662 - 360 WP_000583417.1 holo-ACP synthase -
FOC93_RS20425 3390756..3391328 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
FOC93_RS20430 3391321..3392283 - 963 WP_000961161.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T158347 WP_000635965.1 NZ_CP053951:c3387308-3386958 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T158347 NZ_CP069625:1791-2075 [Escherichia coli]
ATGAGCTATAACCTTGAATTTGATCCCCGAGCCCTGAAGGAATGGCGCAAGCTCGGGGATGATGTCCGTCTGCAGTTCAA
GAAAAAACTCGAGCAGGTTCTACAACACCCGCGGATCGATAAAAATCGCCTGCGAGAGCTGCATGACTGCTACAAAATCA
AGCTCCGTGCATCCGGTTATCGCTTGGTCTATCAGGTTCGCGATCAAACCATTACGGTATTCGTGGTGGCGGTCGGTAAG
CGCGAGCGTTCTGCCGCTTACGATGCGGCCGATAAACGCTTATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158347 WP_000004570.1 NZ_CP053951:c3387600-3387313 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158347 NZ_CP069625:1559-1801 [Escherichia coli]
ATGGCCACGATTAATATTCGGATCGATGACGAGCTGAAAAGCCGCTCTTATGCCGCACTGGAAAAGCTGGGCGTAACGCC
GTCCGAGGTTCTGCGCCAAACACTGGAATATGTGGCCCAAAGCGGACGTTTGCCGTTCCAGCAGGTTTTGCTGACCGAGG
ATGATGCCGATTTGATGGCTATCGTTCGGGATCGTCTGGAAAACCCACAGGCGGGCGTAAAGGTGTCACTGGATGAGCTA
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References