Detailed information of TA system TA158345

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 48222..48750
• Replicon: plasmid unnamed1
• Accession: NZ_CP053948
• Organism: Acinetobacter sp. WY4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G4D73_RS14880
• Protein ID: WP_159373035.1
• Coordinates: 48222..48515 (-)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: N9NWT9
• Locus tag: G4D73_RS14885
• Protein ID: WP_000246755.1
• Coordinates: 48505..48750 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D73_RS14840	43314..44018	-	705	WP_180087571.1	IS6-like element IS1007 family transposase	-
G4D73_RS14845	44089..45369	-	1281	WP_213687790.1	cation transporter	-
G4D73_RS14850	45460..45852	+	393	WP_004733193.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-
G4D73_RS14855	45946..46107	+	162	Protein_56	IS6 family transposase	-
G4D73_RS14860	46149..46451	-	303	WP_001140620.1	XRE family transcriptional regulator	-
G4D73_RS14865	46444..46800	-	357	WP_000269911.1	type II toxin-antitoxin system RelE/ParE family toxin	-
G4D73_RS14870	47364..47699	-	336	WP_129717937.1	hypothetical protein	-
G4D73_RS14875	47746..48138	-	393	WP_213687791.1	hypothetical protein	-
G4D73_RS14880	48222..48515	-	294	WP_159373035.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4D73_RS14885	48505..48750	-	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
G4D73_RS14890	48909..49337	+	429	WP_000877730.1	hypothetical protein	-
G4D73_RS14895	49456..50790	-	1335	WP_042084965.1	ISNCY family transposase	-
G4D73_RS14900	51212..52287	+	1076	WP_213687792.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..165730	165730
	-	inside	IScluster/Tn	-	-	38803..60155	21352

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-84)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 11309.57 Da
• Isoelectric Point: 10.5721

>T158345 WP_159373035.1 NZ_CP053948:c48515-48222 [Acinetobacter sp. WY4]
MTYKLLRHKDFTAEWEKLPVAIRDQFKKKLAKVIEQPHIPKNMLRGDLAGCYKIKLLKAGVRLVYQVKDNQVVILLITVG
KRADSMVYDEAKKRIKD

Nucleotide

• Length: 294 bp

>T158345 NZ_CP069624:4685016-4685396 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATCAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9249.64 Da
• Isoelectric Point: 4.3142

>AT158345 WP_000246755.1 NZ_CP053948:c48750-48505 [Acinetobacter sp. WY4]
MATVNFRIDEALKEKSYSILKEQGIAPTDFFTSILEYVATTGKLPVKKALLSEEDEELLALVRKRINDPKEMFEEVTLDD
L

Nucleotide

• Length: 246 bp

>AT158345 NZ_CP069624:4684595-4684969 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A6A1RJ80