Detailed information of TA system TA158343

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 5913..6501
• Replicon: plasmid unnamed1
• Accession: NZ_CP053948
• Organism: Acinetobacter sp. WY4

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: G4D73_RS14610
• Protein ID: WP_180087573.1
• Coordinates: 5913..6209 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2S4T219
• Locus tag: G4D73_RS14615
• Protein ID: WP_060875721.1
• Coordinates: 6211..6501 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
G4D73_RS14580	1217..3136	+	1920	WP_213687781.1	multicopper oxidase domain-containing protein	-
G4D73_RS14585	3123..3461	+	339	WP_004897649.1	hypothetical protein	-
G4D73_RS14590	3448..4392	+	945	WP_004668309.1	copper resistance protein B	-
G4D73_RS14595	4572..4817	+	246	WP_000246755.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
G4D73_RS14600	4807..5099	+	293	Protein_5	type II toxin-antitoxin system RelE/ParE family toxin	-
G4D73_RS14605	5228..5557	-	330	WP_016658833.1	four-helix bundle copper-binding protein	-
G4D73_RS14610	5913..6209	+	297	WP_180087573.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
G4D73_RS14615	6211..6501	+	291	WP_060875721.1	putative addiction module antidote protein	Antitoxin
G4D73_RS14620	6638..7069	-	432	WP_005264140.1	MarR family transcriptional regulator	-
G4D73_RS14625	7077..7505	-	429	WP_180087572.1	organic hydroperoxide resistance protein	-
G4D73_RS14630	8056..9174	-	1119	WP_213687782.1	Fic family protein	-
G4D73_RS14635	9422..9709	-	288	WP_213687783.1	DUF3861 domain-containing protein	-
G4D73_RS14640	9706..10236	-	531	WP_005254570.1	PadR family transcriptional regulator	-
G4D73_RS14645	10695..10955	-	261	WP_157759226.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..165730	165730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11582.28 Da
• Isoelectric Point: 5.8801

>T158343 WP_180087573.1 NZ_CP053948:5913-6209 [Acinetobacter sp. WY4]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAIKLSKTLED

Nucleotide

• Length: 297 bp

>T158343 NZ_CP069624:c3746968-3746591 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCTTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCAG
CAACTACAGAATGGTGAACGACATCATCCGGGGTGAACATTCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10519.17 Da
• Isoelectric Point: 5.6088

>AT158343 WP_060875721.1 NZ_CP053948:6211-6501 [Acinetobacter sp. WY4]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEDNDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRHDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT158343 NZ_CP069624:c3747389-3747015 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCATCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A2S4T219