Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 1818232..1818787 Replicon chromosome
Accession NZ_CP053940
Organism Bifidobacterium breve strain JTL

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag HQQ90_RS07650 Protein ID WP_003829109.1
Coordinates 1818464..1818787 (+) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag HQQ90_RS07645 Protein ID WP_003829112.1
Coordinates 1818232..1818477 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQQ90_RS07625 1814072..1814734 + 663 WP_042994114.1 TetR/AcrR family transcriptional regulator -
HQQ90_RS07630 1814891..1816162 + 1272 WP_042994115.1 MFS transporter -
HQQ90_RS07635 1816346..1816537 - 192 WP_003829118.1 hypothetical protein -
HQQ90_RS07640 1816802..1818058 - 1257 WP_016463167.1 HipA domain-containing protein -
HQQ90_RS07645 1818232..1818477 + 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
HQQ90_RS07650 1818464..1818787 + 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
HQQ90_RS07655 1819004..1819618 - 615 WP_014483846.1 cupin domain-containing protein -
HQQ90_RS07660 1819712..1820581 - 870 WP_003829104.1 class I SAM-dependent methyltransferase -
HQQ90_RS07665 1820819..1821184 + 366 WP_013140585.1 YccF domain-containing protein -
HQQ90_RS07670 1821726..1822991 + 1266 WP_014483848.1 Fic family protein -
HQQ90_RS07675 1823462..1823760 + 299 Protein_1480 IS256 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1794303..1829018 34715


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T158340 WP_003829109.1 NZ_CP053940:1818464-1818787 [Bifidobacterium breve]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T158340 NZ_CP069624:3442279-3442566 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT158340 WP_003829112.1 NZ_CP053940:1818232-1818477 [Bifidobacterium breve]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT158340 NZ_CP069624:3442553-3442855 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References