Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4686336..4686978 Replicon chromosome
Accession NZ_CP053938
Organism Bacillus thuringiensis strain FDAARGOS_792

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC86_RS24790 Protein ID WP_000635963.1
Coordinates 4686628..4686978 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC86_RS24785 Protein ID WP_000004570.1
Coordinates 4686336..4686623 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC86_RS24760 4681652..4682614 + 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -
FOC86_RS24765 4682607..4683179 - 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC86_RS24770 4683272..4683631 + 360 WP_000635040.1 holo-ACP synthase -
FOC86_RS24775 4683788..4684738 + 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC86_RS24780 4684857..4686026 + 1170 WP_000390600.1 alanine racemase -
FOC86_RS24785 4686336..4686623 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC86_RS24790 4686628..4686978 + 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC86_RS24795 4687046..4689214 + 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC86_RS24800 4689272..4689388 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC86_RS24805 4689584..4690042 + 459 WP_000344247.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158338 WP_000635963.1 NZ_CP053938:4686628-4686978 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158338 NZ_CP069624:3172060-3172233 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158338 WP_000004570.1 NZ_CP053938:4686336-4686623 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158338 NZ_CP069624:3171736-3172032 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References