Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /SpoIISA(toxin)
Location 1568060..1569035 Replicon chromosome
Accession NZ_CP053938
Organism Bacillus thuringiensis strain FDAARGOS_792

Toxin (Protein)


Gene name spoIISA Uniprot ID C3GJ28
Locus tag FOC86_RS08490 Protein ID WP_003297560.1
Coordinates 1568060..1568797 (+) Length 246 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID R8HWP4
Locus tag FOC86_RS08495 Protein ID WP_000588712.1
Coordinates 1568910..1569035 (+) Length 42 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC86_RS08470 1563788..1564570 + 783 WP_000381788.1 class I SAM-dependent methyltransferase -
FOC86_RS08475 1564692..1566377 - 1686 WP_003297558.1 alpha-keto acid decarboxylase family protein -
FOC86_RS08480 1566484..1566966 + 483 WP_000191912.1 MarR family winged helix-turn-helix transcriptional regulator -
FOC86_RS08485 1567134..1567871 + 738 WP_000594156.1 2,3-diphosphoglycerate-dependent phosphoglycerate mutase -
FOC86_RS08490 1568060..1568797 + 738 WP_003297560.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
FOC86_RS08495 1568910..1569035 + 126 WP_000588712.1 hypothetical protein Antitoxin
FOC86_RS08500 1569112..1569288 + 177 WP_000808045.1 hypothetical protein -
FOC86_RS08505 1569432..1570901 + 1470 WP_000287514.1 beta-Ala-His dipeptidase -
FOC86_RS08510 1571136..1571606 - 471 WP_000670597.1 DUF5065 family protein -
FOC86_RS08515 1571783..1572403 + 621 WP_144405038.1 SGNH/GDSL hydrolase family protein -
FOC86_RS08520 1572625..1573158 + 534 WP_000438338.1 sigma-70 family RNA polymerase sigma factor -
FOC86_RS08525 1573148..1573975 + 828 WP_001060391.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(9-243)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 246 a.a.        Molecular weight: 28373.07 Da        Isoelectric Point: 8.2672

>T158337 WP_003297560.1 NZ_CP053938:1568060-1568797 [Bacillus thuringiensis]
VISNIRIGLFILAIVFLILVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSTIIPLEYIEQLNEQRAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Download         Length: 738 bp

>T158337 NZ_CP069624:2864231-2864542 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 42 a.a.        Molecular weight: 4938.34 Da        Isoelectric Point: 3.9150

>AT158337 WP_000588712.1 NZ_CP053938:1568910-1569035 [Bacillus thuringiensis]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Download         Length: 126 bp

>AT158337 NZ_CP069624:2864543-2864833 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3R9EK80

Antitoxin

Source ID Structure
AlphaFold DB A0A366GQT1

References