Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 4689676..4690318 Replicon chromosome
Accession NZ_CP053934
Organism Bacillus thuringiensis strain FDAARGOS_794

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC88_RS24625 Protein ID WP_000635963.1
Coordinates 4689676..4690026 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC88_RS24630 Protein ID WP_000004570.1
Coordinates 4690031..4690318 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC88_RS24610 4686612..4687070 - 459 WP_000344248.1 SprT family protein -
FOC88_RS24615 4687266..4687382 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC88_RS24620 4687440..4689608 - 2169 WP_000426225.1 RNA-binding transcriptional accessory protein -
FOC88_RS24625 4689676..4690026 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC88_RS24630 4690031..4690318 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC88_RS24635 4690628..4691797 - 1170 WP_000390600.1 alanine racemase -
FOC88_RS24640 4691916..4692866 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC88_RS24645 4693023..4693382 - 360 WP_000635040.1 holo-ACP synthase -
FOC88_RS24650 4693475..4694047 + 573 WP_042511773.1 rhomboid family intramembrane serine protease -
FOC88_RS24655 4694040..4695002 - 963 WP_000961155.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158336 WP_000635963.1 NZ_CP053934:c4690026-4689676 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158336 NZ_CP069624:c2447851-2447744 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158336 WP_000004570.1 NZ_CP053934:c4690318-4690031 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158336 NZ_CP069624:2447899-2447965 [Escherichia coli]
GGTCTAGGTTCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References