Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 1373085..1373727 Replicon chromosome
Accession NZ_CP053931
Organism Bacillus cereus strain FDAARGOS_797

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag FOC91_RS08160 Protein ID WP_000635963.1
Coordinates 1373085..1373435 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag FOC91_RS08165 Protein ID WP_000004570.1
Coordinates 1373440..1373727 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FOC91_RS08145 1370021..1370479 - 459 WP_000344244.1 SprT family protein -
FOC91_RS08150 1370675..1370791 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
FOC91_RS08155 1370849..1373017 - 2169 WP_042513310.1 RNA-binding transcriptional accessory protein -
FOC91_RS08160 1373085..1373435 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
FOC91_RS08165 1373440..1373727 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
FOC91_RS08170 1374037..1375206 - 1170 WP_000390600.1 alanine racemase -
FOC91_RS08175 1375325..1376275 - 951 WP_025388382.1 outer membrane lipoprotein carrier protein LolA -
FOC91_RS08180 1376432..1376791 - 360 WP_000635040.1 holo-ACP synthase -
FOC91_RS08185 1376884..1377456 + 573 WP_000906926.1 rhomboid family intramembrane serine protease -
FOC91_RS08190 1377449..1378411 - 963 WP_000961157.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T158333 WP_000635963.1 NZ_CP053931:c1373435-1373085 [Bacillus cereus]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T158333 NZ_CP069624:c1661140-1660733 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT158333 WP_000004570.1 NZ_CP053931:c1373727-1373440 [Bacillus cereus]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT158333 NZ_CP069624:c1661387-1661121 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References