Detailed information of TA system TA158328

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3068638..3069158
• Replicon: chromosome
• Accession: NZ_CP053928
• Organism: Nocardioides sp. WS12

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: HRC28_RS14920
• Protein ID: WP_182376282.1
• Coordinates: 3068856..3069158 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: HRC28_RS14915
• Protein ID: WP_182376281.1
• Coordinates: 3068638..3068856 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRC28_RS14895	3064435..3065304	+	870	WP_182376277.1	LLM class F420-dependent oxidoreductase	-
HRC28_RS14900	3065326..3066969	+	1644	WP_182376278.1	ATP-binding cassette domain-containing protein	-
HRC28_RS14905	3066972..3067364	-	393	WP_182376279.1	VOC family protein	-
HRC28_RS14910	3067442..3068605	+	1164	WP_182376280.1	sterol desaturase family protein	-
HRC28_RS14915	3068638..3068856	+	219	WP_182376281.1	hypothetical protein	Antitoxin
HRC28_RS14920	3068856..3069158	+	303	WP_182376282.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
HRC28_RS14925	3069224..3070021	-	798	WP_182376283.1	hypothetical protein	-
HRC28_RS14930	3070014..3070562	-	549	WP_202033083.1	sigma-70 family RNA polymerase sigma factor	-
HRC28_RS14935	3070559..3070939	-	381	WP_202033084.1	TraR/DksA family transcriptional regulator	-
HRC28_RS14940	3070948..3071316	-	369	WP_182376284.1	hypothetical protein	-
HRC28_RS14945	3071424..3072323	-	900	WP_182376285.1	hypothetical protein	-
HRC28_RS14950	3072320..3072817	-	498	WP_182376286.1	SigE family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-95)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10826.56 Da
• Isoelectric Point: 8.5734

>T158328 WP_182376282.1 NZ_CP053928:3068856-3069158 [Nocardioides sp. WS12]
MRDIHIAHLDKARPVLVLTREPVRGAMRRVTVALITSTAKGLSTEVPVGPANGLEESCVVSCDNIITIDKRSLGRHVGFF
FDRQEADLAAAIVSAFALRS

Nucleotide

• Length: 303 bp

>T158328 NZ_CP069624:c398025-397923 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7651.45 Da
• Isoelectric Point: 3.8083

>AT158328 WP_182376281.1 NZ_CP053928:3068638-3068856 [Nocardioides sp. WS12]
MSVQIAVRLPEELVAYVDALVTDGAGSRAAVVARALGLYQQQLSAERDAQILESSGDYDDFDDLVSHVAVGD

Nucleotide

• Length: 219 bp

>AT158328 NZ_CP069624:397885-398153 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT