Detailed information of TA system TA158326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 443577..444220
• Replicon: chromosome
• Accession: NZ_CP053928
• Organism: Nocardioides sp. WS12

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HRC28_RS02025
• Protein ID: WP_182378480.1
• Coordinates: 443577..443957 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: HRC28_RS02030
• Protein ID: WP_182378482.1
• Coordinates: 443954..444220 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRC28_RS02015	438966..440270	-	1305	WP_182378476.1	aldehyde dehydrogenase family protein	-
HRC28_RS02020	440372..443389	-	3018	WP_182378478.1	S8 family serine peptidase	-
HRC28_RS02025	443577..443957	-	381	WP_182378480.1	type II toxin-antitoxin system VapC family toxin	Toxin
HRC28_RS02030	443954..444220	-	267	WP_182378482.1	ribbon-helix-helix domain-containing protein	Antitoxin
HRC28_RS02035	444252..445793	-	1542	WP_182378484.1	hypothetical protein	-
HRC28_RS02040	445768..446484	-	717	WP_182378487.1	ABC transporter ATP-binding protein	-
HRC28_RS02045	446569..448077	-	1509	WP_182378489.1	CoA-acylating methylmalonate-semialdehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (4-39)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13548.46 Da
• Isoelectric Point: 4.8500

>T158326 WP_182378480.1 NZ_CP053928:c443957-443577 [Nocardioides sp. WS12]
VTVVVDTSVLIDHLRGKSEARVALSALVESGEAMVGTVLTRTEILAGMRSSEEAGTLALLDTFEWVPVTVDIADRAGRFA
QTYLRSHAGVDTVDYVIAATVEAVGGRLWTRNVKHFPMFDGLAAPY

Nucleotide

• Length: 381 bp

>T158326 NZ_CP069624:c68761-68483 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGTAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9764.89 Da
• Isoelectric Point: 4.8550

>AT158326 WP_182378482.1 NZ_CP053928:c444220-443954 [Nocardioides sp. WS12]
MSHRTQITLEDEQYARLRAESAHTGLGLAELVRRAIDEVYGSSTVTERQLRLEASFGAWAIVEMGDDVDGAEYVEKLRGG
LGSRLARQ

Nucleotide

• Length: 267 bp

>AT158326 NZ_CP069624:c68483-68199 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCTACGGCAAGTCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTGTCTGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA