Detailed information of TA system TA158307

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 83599..84365
• Replicon: chromosome
• Accession: NZ_CP053927
• Organism: Serratia marcescens strain 11/2010

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A0P0Q8U6
• Locus tag: HP437_RS01175
• Protein ID: WP_060558096.1
• Coordinates: 83997..84365 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: HP437_RS01170
• Protein ID: WP_180547981.1
• Coordinates: 83599..83934 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HP437_RS01140	78948..79169	-	222	WP_180547977.1	AlpA family transcriptional regulator	-
HP437_RS01145	79631..80488	+	858	WP_180547978.1	hypothetical protein	-
HP437_RS01150	80720..81475	+	756	WP_180548650.1	50S ribosome-binding GTPase	-
HP437_RS01155	81723..82277	+	555	WP_145578351.1	hypothetical protein	-
HP437_RS01160	82305..82925	+	621	WP_180547979.1	hypothetical protein	-
HP437_RS01165	83108..83587	+	480	WP_180547980.1	DNA repair protein RadC	-
HP437_RS01170	83599..83934	+	336	WP_180547981.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
HP437_RS01175	83997..84365	+	369	WP_060558096.1	TA system toxin CbtA family protein	Toxin
HP437_RS01180	84588..85442	+	855	WP_180548651.1	DUF4942 domain-containing protein	-
HP437_RS01185	85732..86244	+	513	WP_149571863.1	DUF962 domain-containing protein	-
HP437_RS01190	86246..86989	+	744	WP_180547982.1	nucleotidyltransferase domain-containing protein	-
HP437_RS01195	87164..88330	+	1167	WP_180547983.1	MFS transporter	-
HP437_RS01200	88515..88751	+	237	WP_019452598.1	DUF4282 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	70164..85442	15278

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-111)

Antitoxin

• Antitoxin CbeA (8-106)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13663.64 Da
• Isoelectric Point: 6.7085

>T158307 WP_060558096.1 NZ_CP053927:83997-84365 [Serratia marcescens]
MQTLPVTPKRAAYACPSPVVVWQSMLTYLLEQHYGLALNDTEFSDDTVIHEHIDAGISLADALNFTVEKFGLVRTDRRGF
SCQEQSPFITAIDILRARRATGLMTRMGYQTITSVIRGEKQA

Nucleotide

• Length: 369 bp

>T158307 NZ_CP069621:727100-727474 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12298.93 Da
• Isoelectric Point: 5.4836

>AT158307 WP_180547981.1 NZ_CP053927:83599-83934 [Serratia marcescens]
MPSIIAPVWGLKRNVTPQFGARLVQEGGRLHFLADRADISGTFSDEQLRDVDNAFPQFINHLELMLLSGELNPRYQHCVT
LYCNGLTCEADSLGSHGYVYLAIYPTPSSTA

Nucleotide

• Length: 336 bp

>AT158307 NZ_CP069621:726643-727011 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• AlphaFold DB: A0A0P0Q8U6