Detailed information of TA system TA158278

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/HTH_IclR(antitoxin)
• Location: 1637917..1639612
• Replicon: chromosome
• Accession: NZ_CP053922
• Organism: Pseudomonas aeruginosa strain YD001

Toxin (Protein)

• Gene name: doc
• Uniprot ID: Q9HYA0
• Locus tag: HQN50_RS07695
• Protein ID: WP_003112901.1
• Coordinates: 1637917..1638786 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: Q9HYA1
• Locus tag: HQN50_RS07700
• Protein ID: WP_003112902.1
• Coordinates: 1638779..1639612 (+)
• Length: 278 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN50_RS07670	1633925..1634776	+	852	WP_003130104.1	ABC transporter ATP-binding protein	-
HQN50_RS07675	1634817..1635824	+	1008	WP_023088129.1	ABC transporter substrate-binding protein	-
HQN50_RS07680	1635821..1636597	+	777	WP_003112898.1	ABC transporter permease	-
HQN50_RS07685	1636603..1637364	+	762	WP_003112899.1	SDR family oxidoreductase	-
HQN50_RS07690	1637378..1637908	+	531	WP_003112900.1	cupin domain-containing protein	-
HQN50_RS07695	1637917..1638786	+	870	WP_003112901.1	alpha/beta hydrolase	Toxin
HQN50_RS07700	1638779..1639612	+	834	WP_003112902.1	IclR family transcriptional regulator	Antitoxin
HQN50_RS07705	1639599..1640396	+	798	WP_017001845.1	SDR family oxidoreductase	-
HQN50_RS07710	1640389..1642071	+	1683	WP_003130114.1	thiamine pyrophosphate-binding protein	-
HQN50_RS07715	1642082..1642885	+	804	WP_003130116.1	aspartate dehydrogenase	-
HQN50_RS07720	1642897..1644381	+	1485	WP_003453562.1	aldehyde dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Alpha/beta hydrolase fold-1 (41-272)

Antitoxin

• Transcription regulator IclR, N-terminal (16-66)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 30403.60 Da
• Isoelectric Point: 6.5894

>T158278 WP_003112901.1 NZ_CP053922:1637917-1638786 [Pseudomonas aeruginosa]
MNMAQTHTPVPNLQQRFPERLVQLADGAQLAIRECGQGPVVVLLHGIGSGSASWLHCAQRLAAGNRVIAWDAPGYGLSTP
LPPARPKACDYAACLELLLDALGVESCLLVGHSLGALMATAYAAGIGAARVRRLVLLSPARGYGAAELRDSGAQVRRQRL
ENLERFGIDGMASERTARLLGRNPSEEALAWVRWNMARLNPEGYRQAVELLCGDDLLGNGQPAAPCEVHCGEDDGITTPE
SCGAIARQLGASFSSIPGAGHASPIEQPEVVAGRIGHAQRLSLEGTANG

Nucleotide

• Length: 870 bp

>T158278 NZ_CP069603:1862994-1863308 [Dickeya dianthicola]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTACCCGGCAGGCCGCCGGCCGGATCGCCTAGTCC
CTGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCTCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATTGACGGTATTTAG

Antitoxin

Protein

• Length: 278 a.a.
• Molecular weight: 30877.26 Da
• Isoelectric Point: 5.7725

>AT158278 WP_003112902.1 NZ_CP053922:1638779-1639612 [Pseudomonas aeruginosa]
MDKSDDSQDKYIVPGLERGLLLLCEFSRKDRTLTAPELARRLKLPRSTIFRLLTTLEAMGFVTRNGNEYRLGMAVLRLGF
EYLASLELTELGQPLLNRLCDEIRYPCNLVVRDGRSIVYVAKVSPSTPLSSSVNVGTRLPAHATVLGRILLQDLSLGELR
ELYPEEQLEQFSPNTPRSVLELFDMVQGDRQRGFVQGEGFFEASISTVAAPVRDHSGRVIAAMGATIAAGHIDPERIEGL
VSRVRSSADELSYLLDYRADGQDNVTPIFRSRPHETV

Nucleotide

• Length: 834 bp

>AT158278 NZ_CP069603:1862755-1862991 [Dickeya dianthicola]
ATGACCGCGAAACAGCGCAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAGGCGGG
CATTAACCTGAGTGCCACTCTGACAACCGCCCTGGATGCCGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAATCGTTTTCATGATGAGCACGGCTGTTTCAGCGATGAATACCGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: Q9HYA0

Antitoxin

• AlphaFold DB: Q9HYA1