Detailed information of TA system TA158272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 822703..823304
• Replicon: chromosome
• Accession: NZ_CP053920
• Organism: Comamonas thiooxydans strain QYY

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HQM26_RS03700
• Protein ID: WP_003055100.1
• Coordinates: 822703..823089 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: B7WTR2
• Locus tag: HQM26_RS03705
• Protein ID: WP_003055097.1
• Coordinates: 823089..823304 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQM26_RS03675	818113..818478	+	366	WP_001277463.1	mercury resistance co-regulator MerD	-
HQM26_RS03680	818475..818711	+	237	WP_003055146.1	broad-spectrum mercury transporter MerE	-
HQM26_RS03685	818777..818989	+	213	WP_000993245.1	DUF3330 domain-containing protein	-
HQM26_RS03690	819119..819679	+	561	WP_003055107.1	recombinase family protein	-
HQM26_RS03695	819682..822650	+	2969	Protein_730	Tn3 family transposase	-
HQM26_RS03700	822703..823089	-	387	WP_003055100.1	type II toxin-antitoxin system VapC family toxin	Toxin
HQM26_RS03705	823089..823304	-	216	WP_003055097.1	DUF2191 domain-containing protein	Antitoxin
HQM26_RS03710	823390..824214	+	825	WP_003055094.1	SprT-like domain-containing protein	-
HQM26_RS03715	824529..827033	-	2505	WP_081007841.1	heavy metal translocating P-type ATPase	-
HQM26_RS03720	827318..828244	-	927	WP_003071641.1	copper homeostasis membrane protein CopD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	676285..959357	283072

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13816.18 Da
• Isoelectric Point: 5.9593

>T158272 WP_003055100.1 NZ_CP053920:c823089-822703 [Comamonas thiooxydans]
MPVSVLVDTSVWVRHFREADIHLQELLLQDSVLMHPMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLAFLEKNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLAELAAQLGVAYAIPTH

Nucleotide

• Length: 387 bp

>T158272 NZ_CP069603:c981700-981395 [Dickeya dianthicola]
ATGCAATTCATTGTTTACCAATACAAACGAGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATTCCGCTTATAGAATCGCATCACCTGTCTGAGAAGGTGAATAAAACGCTGTTCCCTCAGA
TCCGGATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTTCCCGTTGAAGTTATTGGTGAAGAGCTC
GCGGAGCTTGGCGACTATGCGGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7448.46 Da
• Isoelectric Point: 6.2846

>AT158272 WP_003055097.1 NZ_CP053920:c823304-823089 [Comamonas thiooxydans]
MRSTFTIDDEVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGSAPDMPDVPRRGSAAEAEGSR

Nucleotide

• Length: 216 bp

>AT158272 NZ_CP069603:c981921-981703 [Dickeya dianthicola]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAACTATCAGATTCTGAGTGCTGCCGGAGTCAATATTTCTGGTCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGCAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAAAATGGCTCGTTTGCTGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4Y8TDR8