Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 9343..9920 Replicon plasmid pYPR31
Accession NZ_CP053897
Organism Providencia rettgeri strain YPR31

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag HRD55_RS21385 Protein ID WP_023159957.1
Coordinates 9588..9920 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag HRD55_RS21380 Protein ID WP_023159984.1
Coordinates 9343..9588 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRD55_RS21340 4356..4700 + 345 WP_023159966.1 single-stranded DNA-binding protein -
HRD55_RS21345 4693..5115 + 423 WP_159242432.1 hypothetical protein -
HRD55_RS21350 5125..5517 + 393 WP_048821774.1 hypothetical protein -
HRD55_RS21355 5798..6277 + 480 WP_159242433.1 Hcp family type VI secretion system effector -
HRD55_RS21360 6286..6864 + 579 WP_048822182.1 hypothetical protein -
HRD55_RS21365 7625..7993 + 369 WP_048821769.1 hypothetical protein -
HRD55_RS21370 8091..8318 - 228 WP_048821767.1 hypothetical protein -
HRD55_RS21375 8435..9058 - 624 WP_023159958.1 AAA family ATPase -
HRD55_RS21380 9343..9588 + 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HRD55_RS21385 9588..9920 + 333 WP_023159957.1 endoribonuclease MazF Toxin
HRD55_RS21390 9951..10331 + 381 WP_048821763.1 hypothetical protein -
HRD55_RS21395 10420..10560 - 141 WP_072022937.1 type I toxin-antitoxin system Hok family toxin -
HRD55_RS21400 10880..11338 - 459 WP_048821762.1 hypothetical protein -
HRD55_RS21405 11437..11691 - 255 WP_048821760.1 hypothetical protein -
HRD55_RS21410 11730..11999 - 270 WP_048821758.1 hypothetical protein -
HRD55_RS21415 12293..13246 + 954 WP_048821757.1 site-specific integrase -
HRD55_RS21420 13515..14336 + 822 WP_001029679.1 TnsA endonuclease N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid msr(E) / mph(E) / aph(3')-Ia / sul1 / blaDHA-1 / qacE / ARR-3 / aac(6')-Ib-cr / floR / aph(4)-Ia / aac(3)-IVa / blaNDM-1 / ant(3'')-Ia / dfrA1 / lnu(F) - 1..135614 135614


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T158258 WP_023159957.1 NZ_CP053897:9588-9920 [Providencia rettgeri]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T158258 NZ_CP069601:2369367-2369837 [Dickeya dianthicola]
TTGATTCTCTACAGGCTGACCAAGACAAAATATCTTTCTACAGTCTGGACGGGATATGGGGCAAAAGAGGCAGGAGGTCG
CTGGAACAGTGTAGGTGTCTCGATGGTATATGTGTCTGAAACAGCATCGCTTACGATGCTTGAGACGCTTGTACATCTGC
ATGCTGCACATATTCTGGATTATTATACTCTGCTGCGTATTGATGTCCCTGATGACCATATTCAGAGCGTCAATATGAAC
GACTTGCCAGAAAATTGGGCTGAAGAAGACGCGCCGCCTGAGCTTGCTGCTTACGGTGATGCTTGGTGCTTTACCAAAAG
TTCGATAGCCTTGCGTGTCCCCAGCACTTTATCTCCCGTGGAATTTAACTACCTTTTAAACCCCGAGCACCCTGACTTCT
CCTCGATCATCCAGAACGCTGACACCATTCCGTTCCAGTTTGATAGCAGGCTGAAACCGGCGATTAAATAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT158258 WP_023159984.1 NZ_CP053897:9343-9588 [Providencia rettgeri]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT158258 NZ_CP069601:2368927-2369370 [Dickeya dianthicola]
ATGAAAACATTTAGCTTATCCACGACACAGACCCGGCCGCCACGCTTGTGGCAGGTTGCGGGGTTAAACAATTCTGATGG
TGTAGCACTGCTGGGCCAGATTAACGAAGGCCTTGATGGCACAGTTGCAAACGTGATCATTGACTGGGCAAAGATCACGC
AGAACGACCTGCGCAAAATGTCCGGTATACCGTCCACGACATTCAGTCGTAGCCGGAAAGCCCGATTCACCGCCGACCAA
AGTGAGCGACTGGTGCGCATTATTCGCGTTATAGATCGGGCTGTAGAACTGTTCGAGGGTGATAAAGACGAGGCCCAGAA
ATGGTTGAATGAGCCAAACAGAGCACTGAGCTGGAAAGTGCCAGCGGAACTGATGGCCTCCGAAACCGGCGCTTACGAGG
TCATGAAATTGATTACACGCCTGGAGCATGGGGCATACTCTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References