Detailed information of TA system TA158256

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 4501160..4501743
• Replicon: chromosome
• Accession: NZ_CP053896
• Organism: Providencia rettgeri strain YPR31

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: HRD55_RS20840
• Protein ID: WP_096863599.1
• Coordinates: 4501441..4501743 (-)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HRD55_RS20835
• Protein ID: WP_004265229.1
• Coordinates: 4501160..4501444 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HRD55_RS20810	4496320..4497297	-	978	WP_004265237.1	6-phosphofructokinase	-
HRD55_RS20815	4497704..4498648	-	945	WP_004265235.1	phosphatidate cytidylyltransferase	-
HRD55_RS20820	4498645..4499286	-	642	WP_094961883.1	1-acyl-sn-glycerol-3-phosphate acyltransferase	-
HRD55_RS20825	4499524..4500276	-	753	WP_004265231.1	M48 family metallopeptidase	-
HRD55_RS20830	4500496..4501077	-	582	WP_172767500.1	HutD family protein	-
HRD55_RS20835	4501160..4501444	-	285	WP_004265229.1	putative addiction module antidote protein	Antitoxin
HRD55_RS20840	4501441..4501743	-	303	WP_096863599.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HRD55_RS20845	4502000..4502572	-	573	WP_004265226.1	Spy/CpxP family protein refolding chaperone	-
HRD55_RS20850	4502723..4503421	+	699	WP_004265224.1	envelope stress response regulator transcription factor CpxR	-
HRD55_RS20855	4503418..4504788	+	1371	WP_004265222.1	envelope stress sensor histidine kinase CpxA	-
HRD55_RS20860	4505260..4506696	+	1437	WP_136135341.1	capsule assembly Wzi family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-93)

Antitoxin

• Uncharacterized protein HI1420 (3-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11433.37 Da
• Isoelectric Point: 10.0723

>T158256 WP_096863599.1 NZ_CP053896:c4501743-4501441 [Providencia rettgeri]
MITVLTTECFDSWIKNLRDIRAKTKILMRIRRLKNGNYGDVQPIGDGFSELRVHEGQGYRVYLKQKNNVIVILLCGGTKA
TQQKDIHKAKLLFGEVEGEL

Nucleotide

• Length: 303 bp

>T158256 NZ_CP069601:1862994-1863308 [Dickeya dianthicola]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTACCCGGCAGGCCGCCGGCCGGATCGCCTAGTCC
CTGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCTCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATTGACGGTATTTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10454.13 Da
• Isoelectric Point: 4.5983

>AT158256 WP_004265229.1 NZ_CP053896:c4501444-4501160 [Providencia rettgeri]
MNTKLKEFDIAEYLNSEEEIQMYLNEILEEGDPSLVLAALGDIAKARNMSQLAKEVGMSREGLYKALSGQGNPTFMTIIK
IVNSLGLKLQFKSI

Nucleotide

• Length: 285 bp

>AT158256 NZ_CP069601:1862755-1862991 [Dickeya dianthicola]
ATGACCGCGAAACAGCGCAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAGGCGGG
CATTAACCTGAGTGCCACTCTGACAACCGCCCTGGATGCCGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAATCGTTTTCATGATGAGCACGGCTGTTTCAGCGATGAATACCGGACGTTTTAA