Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 39407..39984 Replicon plasmid pJPM24
Accession NZ_CP053895
Organism Proteus mirabilis strain JPM24

Toxin (Protein)


Gene name mazF Uniprot ID U5N651
Locus tag HRD54_RS18520 Protein ID WP_023159957.1
Coordinates 39652..39984 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID U5N340
Locus tag HRD54_RS18515 Protein ID WP_023159984.1
Coordinates 39407..39652 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HRD54_RS18475 34420..34764 + 345 WP_023159966.1 single-stranded DNA-binding protein -
HRD54_RS18480 34757..35179 + 423 WP_159242432.1 hypothetical protein -
HRD54_RS18485 35189..35581 + 393 WP_048821774.1 hypothetical protein -
HRD54_RS18490 35862..36341 + 480 WP_159242433.1 Hcp family type VI secretion system effector -
HRD54_RS18495 36350..36928 + 579 WP_048822182.1 hypothetical protein -
HRD54_RS18500 37689..38057 + 369 WP_048821769.1 hypothetical protein -
HRD54_RS18505 38155..38382 - 228 WP_048821767.1 hypothetical protein -
HRD54_RS18510 38499..39122 - 624 WP_023159958.1 AAA family ATPase -
HRD54_RS18515 39407..39652 + 246 WP_023159984.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
HRD54_RS18520 39652..39984 + 333 WP_023159957.1 endoribonuclease MazF Toxin
HRD54_RS18525 40015..40395 + 381 WP_048821763.1 hypothetical protein -
HRD54_RS18530 40484..40624 - 141 WP_072022937.1 type I toxin-antitoxin system Hok family toxin -
HRD54_RS18535 40944..41402 - 459 WP_048821762.1 hypothetical protein -
HRD54_RS18540 41501..41755 - 255 WP_048821760.1 hypothetical protein -
HRD54_RS18545 41794..42063 - 270 WP_048821758.1 hypothetical protein -
HRD54_RS18550 42357..43310 + 954 WP_048821757.1 site-specific integrase -
HRD54_RS18555 43579..44400 + 822 WP_001029679.1 TnsA endonuclease N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid msr(E) / mph(E) / blaNDM-1 / sul1 / qacE / ant(3'')-Ia / dfrA1 / lnu(F) - 1..102319 102319


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

SpoVT-AbrB domain

(7-49)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 12097.88 Da        Isoelectric Point: 7.0813

>T158248 WP_023159957.1 NZ_CP053895:39652-39984 [Proteus mirabilis]
MVSRYVPDSGDLIWIDFDPVEGHEQGGHRPAVVLSPFAYNNKVGLLLCVPCTTKGKGYPFECELSNERDSYALADQVTCV
DWRARKVTKKGKVEASELAEIKAKAKALIG

Download         Length: 333 bp

>T158248 NZ_CP069601:c474341-473862 [Dickeya dianthicola]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACGCTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGCAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTGCTCGGTAGATTAGCCGTTGATGAACGTTATCAACGTATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCCCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8786.20 Da        Isoelectric Point: 5.0351

>AT158248 WP_023159984.1 NZ_CP053895:39407-39652 [Proteus mirabilis]
MAQVTVKKWGNSPSVRLPVAIMQKAALSVDDTVEIAVEEGRIIITPVKAVEYSLDNLLAGITPENIHEKVDFGARTGKEL
I

Download         Length: 246 bp

>AT158248 NZ_CP069601:c474604-474332 [Dickeya dianthicola]
ATGCGAACAGAGACAAAAGAAACGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCCGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAAAGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGCTGGAATCACCTGTCACAGATAATCCGCGTATGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P1BQ75

Antitoxin

Source ID Structure
AlphaFold DB A0A857E7M9

References