Detailed information of TA system TA158232

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4162478..4164192
• Replicon: chromosome
• Accession: NZ_CP053885
• Organism: Aeromonas hydrophila strain 3019

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: A0A6S5IKK5
• Locus tag: HQ395_RS19640
• Protein ID: WP_049636669.1
• Coordinates: 4163539..4164192 (-)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: HQ395_RS19635
• Protein ID: WP_252347648.1
• Coordinates: 4162478..4163491 (-)
• Length: 338 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ395_RS19620 (HQ395_19620)	4157602..4158540	+	939	WP_171269279.1	restriction endonuclease	-
HQ395_RS19625 (HQ395_19625)	4158559..4159272	-	714	WP_005331303.1	SprT family zinc-dependent metalloprotease	-
HQ395_RS19630 (HQ395_19630)	4159272..4162403	-	3132	WP_182954683.1	HsdR family type I site-specific deoxyribonuclease	-
HQ395_RS19635 (HQ395_19635)	4162478..4163491	-	1014	WP_252347648.1	macro domain-containing protein	Antitoxin
HQ395_RS19640 (HQ395_19640)	4163539..4164192	-	654	WP_049636669.1	DUF4433 domain-containing protein	Toxin
HQ395_RS19645 (HQ395_19645)	4164199..4166235	-	2037	WP_049636668.1	DUF262 domain-containing protein	-
HQ395_RS19650 (HQ395_19650)	4166244..4167734	-	1491	WP_232098005.1	DUF262 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4142661..4218873	76212

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (1-114)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25229.78 Da
• Isoelectric Point: 6.8260

>T158232 WP_049636669.1 NZ_CP053885:c4164192-4163539 [Aeromonas hydrophila]
MGYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQQQSPHWVHIGLPELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPQRENDEILILVSSLHRIAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDAEDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNSVEQCMNRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

>T158232 NZ_CP069599:1578338-1578652 [Dickeya dianthicola]
ATGCAATTCACCGTATACGGTAATACTGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
GCAGTTAAATCGCCGGATAGTGATCCCGTTGCTCCCGGTTGAAAAGTACCCGGCAGGCCGCCGGCCGGATCGCCTAGTCC
CTGTCGTCAGGCTGACGGACGGTAAAGAATACGCCGTAATGGCGCACGAGCTGGCAAGTATCCCTGTGCAGGCTCTGGGG
GCGGTGTTCTGTGATGCGTCGCAGTACCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATTGACGGTATTTAG

Antitoxin

Protein

• Length: 338 a.a.
• Molecular weight: 37729.53 Da
• Isoelectric Point: 6.8626

>AT158232 WP_252347648.1 NZ_CP053885:c4163491-4162478 [Aeromonas hydrophila]
VNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTKQHWRNPAQMAWIQDGLQ
DLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGGVAELTPARALIAELVRR
YWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQSDKRIPDSKPFDVIWFNE
AKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSALREGLHRWPAGTPWGERKARLF
DDRSLQIALTRLQQGLV

Nucleotide

• Length: 1014 bp

>AT158232 NZ_CP069599:1578099-1578335 [Dickeya dianthicola]
ATGACCGCGAAACAACGCAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGCTCCGGGCGCGTGAAGCGGG
TATTAACCTGAGTGCCACCCTGACAACCGCCCTGGATGCAGAGCTTCGCCGTTATGAGGCGAAAAAATGGCAGGAAGAGA
ACCGGGAAGCCATCGACGCATTAAACCGTTTTCATGATGAAAACGGCTGTTTCAGCGATGAATACCGGACGTTTTAA

Structures

Toxin

• AlphaFold DB: A0A6S5IKK5