Detailed information of TA system TA158230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1827977..1829691
• Replicon: chromosome
• Accession: NZ_CP053885
• Organism: Aeromonas hydrophila strain 3019

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: HQ395_RS08865
• Protein ID: WP_025201143.1
• Coordinates: 1827977..1828630 (+)
• Length: 218 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: HQ395_RS08870
• Protein ID: WP_252347648.1
• Coordinates: 1828678..1829691 (+)
• Length: 338 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQ395_RS08855 (HQ395_08855)	1824990..1826126	-	1137	WP_021141311.1	ISAs1-like element ISAs12 family transposase	-
HQ395_RS08860	1826294..1827709	+	1416	WP_215783531.1	restriction endonuclease subunit S	-
HQ395_RS08865 (HQ395_08865)	1827977..1828630	+	654	WP_025201143.1	DUF4433 domain-containing protein	Toxin
HQ395_RS08870 (HQ395_08870)	1828678..1829691	+	1014	WP_252347648.1	macro domain-containing protein	Antitoxin
HQ395_RS08875 (HQ395_08875)	1829766..1832897	+	3132	WP_215783532.1	HsdR family type I site-specific deoxyribonuclease	-
HQ395_RS08880 (HQ395_08880)	1832897..1833610	+	714	WP_215783533.1	SprT family zinc-dependent metalloprotease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1815580..1838668	23088
	-	flank	IS/Tn	-	-	1824990..1826126	1136

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-216)

Antitoxin

• Macro domain (1-114)

Sequences

Toxin

Protein

• Length: 218 a.a.
• Molecular weight: 25252.81 Da
• Isoelectric Point: 6.8796

>T158230 WP_025201143.1 NZ_CP053885:1827977-1828630 [Aeromonas hydrophila]
MAYDYSQSLNPQKALIFRIVHRDNLEWILTHGLWAGNGQHQSPHWVHIGLPELIAKRARHPVPLAPYGVLNDYVPFYFTP
FSPMLSNIHSGRGVPQRENDEILILVSSLHRIAELGLPFVFTDSHAYYQWANFYVDLGDLGRIDWPLLQRRDFKRDAEDL
SKLERYQAEALIHQHCPIEALLGVVCYNEEIKNSVEQCMNRVGVARPVYVRDGWYFR

Nucleotide

• Length: 654 bp

>T158230 NZ_CP069599:c1259149-1258946 [Dickeya dianthicola]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCTGCAGACCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAAATACGTTCGTTAA

Antitoxin

Protein

• Length: 338 a.a.
• Molecular weight: 37729.53 Da
• Isoelectric Point: 6.8626

>AT158230 WP_252347648.1 NZ_CP053885:1828678-1829691 [Aeromonas hydrophila]
VNTVNTVGVMGKGIALMFKERFPANMRAYAEACKQGDVVTGKMFITQTGELLGPRWIVNFPTKQHWRNPAQMAWIQDGLQ
DLRAWLQAQQVSSIAIPPLGAGNGGLPWPEVKQAIETALGDLDIDILLYEPSSQYQNVAKRGGVAELTPARALIAELVRR
YWFLGMECSLLEIQKLAWFLARVIGRNPLGDPLQLQFKAHRYGPYANNLTHLLNAMDGSYLQSDKRIPDSKPFDVIWFNE
AKKQELAVYLQTEAKAFLPILEQTTALIDGFESPYGMELLATVDWLIAEEGCAPTLSALREGLHRWPAGTPWGERKARLF
DDRSLQIALTRLQQGLV

Nucleotide

• Length: 1014 bp

>AT158230 NZ_CP069599:c1259571-1259203 [Dickeya dianthicola]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGATAGCAGTCGTAGCTGGGTGAATGACCCGACTTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCTGCGTTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGTAACTACGGTATAAATGACGCCGAGTTGCAGAAATGGCGAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA