Detailed information of TA system TA158228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 4696..5276
• Replicon: plasmid pn142_2
• Accession: NZ_CP053877
• Organism: Klebsiella pneumoniae strain KPN142

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: HPY03_RS28060
• Protein ID: WP_071177730.1
• Coordinates: 4696..5010 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: HPY03_RS28065
• Protein ID: WP_000093040.1
• Coordinates: 4998..5276 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPY03_RS28035 (HPY03_27960)	640..2604	+	1965	WP_071177727.1	TraM recognition domain-containing protein	-
HPY03_RS28040 (HPY03_27965)	2604..3335	+	732	WP_071177728.1	MobC family replication-relaxation protein	-
HPY03_RS28045 (HPY03_27970)	3342..3872	+	531	WP_071177729.1	hypothetical protein	-
HPY03_RS28050 (HPY03_27975)	3899..4078	-	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
HPY03_RS28055 (HPY03_27980)	4104..4532	-	429	WP_001140599.1	hypothetical protein	-
HPY03_RS28060 (HPY03_27985)	4696..5010	-	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPY03_RS28065 (HPY03_27990)	4998..5276	-	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
HPY03_RS28070	5451..5816	-	366	WP_072354022.1	TonB family protein	-
HPY03_RS28075 (HPY03_28000)	5813..6184	-	372	WP_001237044.1	cell envelope integrity protein TolA	-
HPY03_RS28080 (HPY03_28005)	6458..6703	-	246	WP_032440458.1	hypothetical protein	-
HPY03_RS28085 (HPY03_28010)	7030..8715	+	1686	WP_032440457.1	colicin-like bacteriocin tRNase domain-containing protein	-
HPY03_RS28090 (HPY03_28015)	8725..8982	+	258	WP_032440455.1	colicin E3-like toxin immunity protein	-
HPY03_RS28095 (HPY03_28020)	9067..9216	+	150	WP_032440454.1	colicin release lysis protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T158228 WP_071177730.1 NZ_CP053877:c5010-4696 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T158228 NZ_CP069599:c981704-981399 [Dickeya dianthicola]
ATGCAATTCATTGTTTACCAATACAAACGAGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATTCCGCTTATAGAATCGCATCACCTGTCTGAGAAGGTGAATAAAACGCTGTTCCCTCAGA
TCCGGATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTTCCCGTTGAAGTTATTGGTGAAGAGCTC
GCGGAGCTTGGCGACTATGCGGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT158228 WP_000093040.1 NZ_CP053877:c5276-4998 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT158228 NZ_CP069599:c981925-981707 [Dickeya dianthicola]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAACTATCAGATTCTGAGTGCTGCCGGAGTCAATATTTCTGGTCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGCAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAAAATGGCTCGTTTGCTGATGAAAACAGGAACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1