158226

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5388044..5388669 Replicon chromosome
Accession NZ_CP053875
Organism Klebsiella pneumoniae strain KPN142

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag HPY03_RS26620 Protein ID WP_002882817.1
Coordinates 5388044..5388427 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag HPY03_RS26625 Protein ID WP_004150355.1
Coordinates 5388427..5388669 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY03_RS26605 (HPY03_26555) 5385410..5386312 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
HPY03_RS26610 (HPY03_26560) 5386309..5386944 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
HPY03_RS26615 (HPY03_26565) 5386941..5387870 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
HPY03_RS26620 (HPY03_26570) 5388044..5388427 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
HPY03_RS26625 (HPY03_26575) 5388427..5388669 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
HPY03_RS26630 (HPY03_26580) 5388874..5389791 + 918 WP_002882812.1 alpha/beta hydrolase -
HPY03_RS26635 (HPY03_26585) 5389805..5390746 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
HPY03_RS26640 (HPY03_26590) 5390791..5391228 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
HPY03_RS26645 (HPY03_26595) 5391225..5392085 - 861 WP_002882807.1 virulence factor BrkB family protein -
HPY03_RS26650 (HPY03_26600) 5392079..5392678 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T158226 WP_002882817.1 NZ_CP053875:c5388427-5388044 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T158226 NZ_CP069599:c474345-473866 [Dickeya dianthicola]
GTGGGAATAACGGCACCTGAATTATTGCTGCCTCAACATGCGGTTGTGGATTTTCATTGTTCAGAGCCGTCATTGAATGA
ATGGCTGAAACGCAAGGCGTTAAAAAATCAGACGCTGGGCGCTTCACGCACCTTCGTGGTGTGTGAAGCCGGTACACAGC
GTGTGGTGGGGTTCTATGCCCTCGCGTCGGGCAGCATCCAGCGTCAGGTTGCGCCGGGCGCATTCCGGCGCAATATGCCT
GACCCCATTCCTGTTCTGGTGCTCGGTAGATTAGCCGTTGATGAACGTTATCAACGTATGGGGATCGGTGCCGGGCTCCT
GAAAGATGCGGTACTCCGCTCCCGCAATGTAGCGCAGCAGGTAGGCAACAAAGCGTTATTGGTACATGCGTTATCTGATG
AGGCAAAAGCGTTCTACCAATACTGGGGTTTTGTCCCGTCAGAAATACAGGAACACACCTTATTATTATCGTTATGGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT158226 WP_004150355.1 NZ_CP053875:c5388669-5388427 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT158226 NZ_CP069599:c474608-474336 [Dickeya dianthicola]
ATGCGAACAGAGACAAAAGAAACGCCGATTAATATTCGTGCTAAAGCATCACAGCGGGAGCTGATTGATGTCGCCGCTAA
ATTGCTGTCGAAATCAAGAACAGAGTTTATCCTTGATGCTGCCTGCCGTGAGGCTGAGGATGTGTTGTTGGATCAAAGGC
TGTTTCTGGTCAATGATGAACAATATGATGCTTTCATTCAGGTGCTGGAATCACCTGTCACAGATAATCCGCGTATGAAT
GCATTACTGAACAGGAAATCTCCGTGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References