158223

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4806694..4807504 Replicon chromosome
Accession NZ_CP053875
Organism Klebsiella pneumoniae strain KPN142

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag HPY03_RS23835 Protein ID WP_002887280.1
Coordinates 4806694..4807227 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag HPY03_RS23840 Protein ID WP_002887278.1
Coordinates 4807238..4807504 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY03_RS23830 (HPY03_23795) 4805525..4806646 + 1122 WP_002887282.1 cupin domain-containing protein -
HPY03_RS23835 (HPY03_23800) 4806694..4807227 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
HPY03_RS23840 (HPY03_23805) 4807238..4807504 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
HPY03_RS23845 (HPY03_23810) 4807607..4809040 - 1434 WP_002887275.1 Cu(+)/Ag(+) sensor histidine kinase -
HPY03_RS23850 (HPY03_23815) 4809030..4809713 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
HPY03_RS23855 (HPY03_23820) 4809885..4811270 + 1386 WP_002887267.1 efflux transporter outer membrane subunit -
HPY03_RS23860 (HPY03_23825) 4811288..4811632 + 345 WP_002887266.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T158223 WP_002887280.1 NZ_CP053875:c4807227-4806694 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T158223 NZ_CP069598:1862994-1863308 [Dickeya dianthicola]
ATGCAATTCACCGTATACGGTAATACCGGGAAAAGCGTCGTTTACCCGCTGTTGCTCGATGTCACGAGCGATATTATTGG
ACAGTTGAATCGTCGGATAGTGATCCCGTTGCTCCCTGTTGAAAAGTACCCGGCAGGCCGCCGGCCGGATCGCCTAGTCC
CTGTCGTCAGGCTGACGGACGGCAAAGAGTACGCCGTAATGACGCACGAGCTGGCAAGTATCCCTGTGCAGGCTCTGGGG
GCGGTGTTTTGTGATGCGTCGCAGTACCGTACTCAGGTAAAGGCCGCAATAGATTTCCTCATTGACGGTATTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT158223 WP_002887278.1 NZ_CP053875:c4807504-4807238 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT158223 NZ_CP069598:1862755-1862991 [Dickeya dianthicola]
ATGACCGCGAAACAGCGCAACACGCAGAGCGTGACCATGACGGTAGAGCGTGCCTTACTGGTCCGGGCGCGTGAGGCGGG
CATTAACCTGAGTGCCACTCTGACAACCGCCCTGGATGCCGAGCTTCGCCGTCATGAAGCGAAAAAATGGCAGGAAGAGA
ACAGGGAAGCCATCGACGCATTAAATCGTTTTCATGATGAGCACGGCTGTTTCAGCGATGAATACCGGACGTTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GLZ1

References