Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5337625..5338250 Replicon chromosome
Accession NZ_CP053862
Organism Klebsiella pneumoniae strain KP-Zhen

Toxin (Protein)


Gene name vapC Uniprot ID A0A330XB05
Locus tag GWF98_RS25560 Protein ID WP_012737091.1
Coordinates 5337625..5338008 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GWF98_RS25565 Protein ID WP_004150355.1
Coordinates 5338008..5338250 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWF98_RS25545 (5334991) 5334991..5335893 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
GWF98_RS25550 (5335890) 5335890..5336525 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GWF98_RS25555 (5336522) 5336522..5337451 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GWF98_RS25560 (5337625) 5337625..5338008 - 384 WP_012737091.1 type II toxin-antitoxin system VapC family toxin Toxin
GWF98_RS25565 (5338008) 5338008..5338250 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
GWF98_RS25570 (5338455) 5338455..5339372 + 918 WP_012737090.1 alpha/beta hydrolase -
GWF98_RS25575 (5339386) 5339386..5340327 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GWF98_RS25580 (5340372) 5340372..5340809 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
GWF98_RS25585 (5340806) 5340806..5341666 - 861 WP_004146232.1 virulence factor BrkB family protein -
GWF98_RS25590 (5341660) 5341660..5342259 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14266.53 Da        Isoelectric Point: 7.2771

>T158197 WP_012737091.1 NZ_CP053862:c5338008-5337625 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRCEAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKGFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T158197 NZ_CP069596:c1259145-1258942 [Dickeya dianthicola]
ATGAAAAAAATCGACTATTTAATGCGTTTGCGTAAATGCACAACCATCGACACGCTTGAGCGTGTTATAGAAAAAAACAA
GTACGAGCTTTCCAACGATGAGCTTGAAATGTTCTATTCAGCTGCAGACCACCGCCTGGCAGAGCTGACGATGAATAAGC
TTTACGACAAAGTACCGACTGCTGTGTGGAAATACGTTCGTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT158197 WP_004150355.1 NZ_CP053862:c5338250-5338008 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT158197 NZ_CP069596:c1259567-1259199 [Dickeya dianthicola]
ATGGATGAGTACACACCTCAACATTATGATATTGCCCAACTCAGATTCTTGTGTGAAAATCTGCACGACGAAAGTATCGC
GACATTAGGTGATAGCAGTCGTAGCTGGGTGAATGACCCGACTTCTGCGGTCAACCTTCAACTAAACGAACTTATCGAGC
ATATTGCGGCATTTGTTGTTACCTACAAAATAAAATACCCGCATGAAGCTGCGTTATGTGAACGGGTTGAGAAATATCTG
GACGACACTTATATTCTTTTCAGTAACTACGGTATAAATGACGCCGAGTTGCAGAAATGGCGAAAATCCAAGTCACAATT
ATTCAGAATGTTCTCAGAAAAGAGCATCTGTACGGTAGTAAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A330XB05

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References