Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4700473..4701283 Replicon chromosome
Accession NZ_CP053862
Organism Klebsiella pneumoniae strain KP-Zhen

Toxin (Protein)


Gene name KacT Uniprot ID A0A332NV98
Locus tag GWF98_RS22585 Protein ID WP_014908042.1
Coordinates 4700473..4701006 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag GWF98_RS22590 Protein ID WP_002887278.1
Coordinates 4701017..4701283 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWF98_RS22580 (4699304) 4699304..4700425 + 1122 WP_012737335.1 cupin domain-containing protein -
GWF98_RS22585 (4700473) 4700473..4701006 - 534 WP_014908042.1 type II toxin-antitoxin system toxin KacT Toxin
GWF98_RS22590 (4701017) 4701017..4701283 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
GWF98_RS22595 (4701386) 4701386..4702819 - 1434 WP_014908043.1 Cu(+)/Ag(+) sensor histidine kinase -
GWF98_RS22600 (4702809) 4702809..4703492 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
GWF98_RS22605 (4703664) 4703664..4705049 + 1386 WP_012737332.1 efflux transporter outer membrane subunit -
GWF98_RS22610 (4705067) 4705067..4705411 + 345 WP_012737331.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(87-150)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19795.68 Da        Isoelectric Point: 5.2614

>T158195 WP_014908042.1 NZ_CP053862:c4701006-4700473 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T158195 NZ_CP069596:c981700-981395 [Dickeya dianthicola]
ATGCAATTCATTGTTTACCAATACAAACGAGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATTCCGCTTATAGAATCGCATCACCTGTCTGAGAAGGTGAATAAAACGCTGTTCCCTCAGA
TCCGGATCGACGGCGAAGATTATCGGCTGATGACGACTGAACTATCGAGCGTTCCCGTTGAAGTTATTGGTGAAGAGCTC
GCGGAGCTTGGCGACTATGCGGATGAGATCAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT158195 WP_002887278.1 NZ_CP053862:c4701283-4701017 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT158195 NZ_CP069596:c981921-981703 [Dickeya dianthicola]
ATGAAACACCGCGTCAGCGTTACCGTGGACAAAGACAACTATCAGATTCTGAGTGCTGCCGGAGTCAATATTTCTGGTCT
GGTGAATGATGCCATTGGCAAAGAAGCCCGCCGTATCAAGGCTGAGCAGTGGAAGAAGGAAAACCGCGAAGGGATGGAAG
AAGTCGCCCGATTTATCGCACAAAATGGCTCGTTTGCTGATGAAAACAGGAACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A332NV98

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References