Detailed information of TA system TA158181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 239549..240129
• Replicon: plasmid pR76
• Accession: NZ_CP053858
• Organism: Rhizobium pusense strain 76

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: HQN82_RS24250
• Protein ID: WP_173616195.1
• Coordinates: 239549..239932 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A5D9CLR1
• Locus tag: HQN82_RS24255
• Protein ID: WP_006699143.1
• Coordinates: 239929..240129 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN82_RS24230	235459..237171	+	1713	WP_173616192.1	ABC transporter ATP-binding protein	-
HQN82_RS24235	237183..237968	+	786	WP_173616193.1	hydroxypyruvate isomerase	-
HQN82_RS24240	237972..238823	+	852	WP_173616194.1	2-hydroxy-3-oxopropionate reductase	-
HQN82_RS24245	239185..239460	+	276	WP_173616403.1	hypothetical protein	-
HQN82_RS24250	239549..239932	-	384	WP_173616195.1	type II toxin-antitoxin system VapC family toxin	Toxin
HQN82_RS24255	239929..240129	-	201	WP_006699143.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
HQN82_RS24260	240310..242667	-	2358	Protein_227	hypothetical protein	-
HQN82_RS24265	242875..243981	+	1107	WP_173616196.1	Fic family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB / htpB	1..486984	486984

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14090.29 Da
• Isoelectric Point: 7.1290

>T158181 WP_173616195.1 NZ_CP053858:c239932-239549 [Rhizobium pusense]
VILVDSSIWIDHFRHVNPELVKIIGDDQLLCHPFVVGELALGSLRERDAVLAFLTAQREAVVATHAEVMTVIDRHSIFSM
GIGYTDAHLLTSTLLDRRSSLWTRDKRLAAAARKVGAALYPSPHIPQ

Nucleotide

• Length: 384 bp

>T158181 NZ_CP069594:c1731559-1731377 [Pseudomonas sp. Y39-6]
GTGGATAGCCGATATTTAATAGGCCATATCGTCGCCGACGGCTGGTATCTGGTGCGCGTCAGAGGTAGCCATCACCCCTT
CAAGCACCCGACGAAACCGGGGCTGGTGACAATTCCTCATCCGAAGAAGGACTTGTTGGACAAGACCGCCAAAAGCATGT
TGAAACAGGCACTGCTGAGTTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7168.28 Da
• Isoelectric Point: 10.7407

>AT158181 WP_006699143.1 NZ_CP053858:c240129-239929 [Rhizobium pusense]
MRSTINLDDSLMERAKSLTGTKETAALVRQALETLVRVESGKRLIALGGTMPEAEAAPRRRSEVGK

Nucleotide

• Length: 201 bp

>AT158181 NZ_CP069594:c1731354-1730944 [Pseudomonas sp. Y39-6]
GTGCTGTACCCCATCGCAATTTCTACTGGTGACGAAGACCATGCCTGGGGCGTGGAAGTCCCGGACATTCCTGGTTGCTA
CTCCGCCGGTGACGACTTGGATGACGCCATGGCCATGGCCCGTGAAGCGATTGAAGGCCACTTCGAAATTCTCGCCGAAG
ACGGTGCGCCGATTCCCAGCGCGCAGAAAGTCACCCTGCATGCAGCCAACCCCAAGTACGCCGGTTGCATGTGGGCAGTG
GTGGACATCGATGTCACCAAATACCTGGGCAAGGCGCAGAAACTCAACATCACCCTGCCAGGCTATTTGCTCAATCGTAT
CGATGAATACGTACTGCACCATCCCGAGGAAAAAAGCCGTTCGGGGTTCCTCGCGTCCGCAGCGCTCAAGGTGCTGCAAC
AGGATCGATAG

Structures

Antitoxin

• AlphaFold DB: A0A5D9CLR1