Detailed information of TA system TA158179

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 489362..489959
• Replicon: chromosome
• Accession: NZ_CP053856
• Organism: Rhizobium pusense strain 76

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A1L9CW50
• Locus tag: HQN82_RS02340
• Protein ID: WP_072494132.1
• Coordinates: 489642..489959 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HQN82_RS02335
• Protein ID: WP_072494131.1
• Coordinates: 489362..489661 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN82_RS02310	484499..485497	+	999	WP_173614499.1	asparaginase	-
HQN82_RS02315	485632..486252	+	621	WP_173614500.1	glutathione S-transferase family protein	-
HQN82_RS02320	486403..487062	-	660	WP_107340977.1	glutathione S-transferase family protein	-
HQN82_RS02325	487145..487660	-	516	WP_004440484.1	MarR family transcriptional regulator	-
HQN82_RS02330	487802..489331	+	1530	WP_006700017.1	MFS transporter	-
HQN82_RS02335	489362..489661	-	300	WP_072494131.1	putative addiction module antidote protein	Antitoxin
HQN82_RS02340	489642..489959	-	318	WP_072494132.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQN82_RS02345	490198..491208	+	1011	WP_173614988.1	ligase-associated DNA damage response exonuclease	-
HQN82_RS02350	491210..492835	+	1626	WP_045532529.1	cisplatin damage response ATP-dependent DNA ligase	-
HQN82_RS02355	493007..493972	+	966	WP_173614501.1	prolyl oligopeptidase family serine peptidase	-
HQN82_RS02360	494058..494840	-	783	WP_162699290.1	mannosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (5-96)

Antitoxin

• Uncharacterized protein HI1420 (4-97)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11847.47 Da
• Isoelectric Point: 9.5802

>T158179 WP_072494132.1 NZ_CP053856:c489959-489642 [Rhizobium pusense]
MTNDFEKSAEFDEWLKALKDRTGKARILHRLTAAEHGNFGDCEPVGGGVSEMRIHCGPGYRVYFTRIGKRVYFLLVGGDK
SSQKRDIKRAVEMAQTIGRNDRDAV

Nucleotide

• Length: 318 bp

>T158179 NZ_CP069594:c685242-684940 [Pseudomonas sp. Y39-6]
ATGATTGAGTTCGAAGAATCCGTTGAGTTTGTCAAATGGCTCGAAGCAATGCGCGATCCGCTCGCAAAAGTACGCATTGT
GACAAGGCTGCGCATGGCGCAAGCAGGAAACTTCGGCGACTGTGAGGCTGTAGGAGAGGGCGTTTGCGAAATGCGAATCC
ACCAAGGCCCCGGGTATCGGGTTTACTTCACTCTCCGAGACAAGGTGGTTTATCTGCTGCTGATGGGCGGCGACAAATCA
ACACAAAGCCGAGACATCCAGCGTGCAATACACATGGCAAAAAATCTTGGCCGCAAGGAGTGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10742.21 Da
• Isoelectric Point: 5.1007

>AT158179 WP_072494131.1 NZ_CP053856:c489661-489362 [Rhizobium pusense]
MTVTQFSKFDVSDYLDSDEAMLEYLAVAMEDENPDVFIAALGAVARARGMSHVADAAGLGRESLYKALKPGSKLRYETVQ
KVLHSLGFRLAVEKSSNAA

Nucleotide

• Length: 300 bp

>AT158179 NZ_CP069594:c684936-684646 [Pseudomonas sp. Y39-6]
ATGAGCGAATCCTTTACCCGTTTCGATGCCGCAAAATACCTTAAGACTCCTTTTGAGATGGCCGCCTATCTGGATGCTTG
CTTTGAAGAAGATGCTGGCGACGGTCTGTTGATTCGCACAGCGTTGAACGATATCGCTCGCGCCCAAGGCATGACCCAAG
TCGCTCGCGACGCCGGCCTAGGACGAGAAAGTTTGTACAAAGCCCTCTCCAGCACTGGCAATCCCGAGTTCGCGACTATC
ATGAAGGTCATGAGAGCACTGGGGCTCAAACTTCACGCCACCGCGCTCTGA

Structures

Toxin

• AlphaFold DB: A0A1L9CW50