Detailed information of TA system TA158178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 444049..444593
• Replicon: chromosome
• Accession: NZ_CP053856
• Organism: Rhizobium pusense strain 76

Toxin (Protein)

• Gene name: parE
• Uniprot ID: U4PRC3
• Locus tag: HQN82_RS02125
• Protein ID: WP_004440407.1
• Coordinates: 444303..444593 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: HQN82_RS02120
• Protein ID: WP_107341193.1
• Coordinates: 444049..444300 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HQN82_RS02095	439147..439836	-	690	WP_003520496.1	HAD family hydrolase	-
HQN82_RS02100	440051..440731	+	681	WP_004440402.1	GntR family transcriptional regulator	-
HQN82_RS02105	440772..441233	+	462	WP_112766665.1	DUF1284 domain-containing protein	-
HQN82_RS02110	441193..442293	-	1101	WP_045532570.1	hypothetical protein	-
HQN82_RS02115	442530..443675	+	1146	WP_004440405.1	site-specific DNA-methyltransferase	-
HQN82_RS02120	444049..444300	+	252	WP_107341193.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
HQN82_RS02125	444303..444593	+	291	WP_004440407.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HQN82_RS02130	444625..445236	-	612	WP_112766511.1	HAD family phosphatase	-
HQN82_RS02135	445233..446336	-	1104	WP_112766510.1	A/G-specific adenine glycosylase	-
HQN82_RS02140	446434..446961	+	528	WP_006699987.1	DUF721 domain-containing protein	-
HQN82_RS02145	447090..447770	+	681	WP_173614487.1	DsbA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (8-62)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11253.77 Da
• Isoelectric Point: 6.6456

>T158178 WP_004440407.1 NZ_CP053856:444303-444593 [Rhizobium pusense]
MGFRLSLPAEEDVIRIAQEGIDLFGPQQARKYHRELFAIFDLISRNPRIARERNELSPSVRIHPFRAHLVVYRVEADDDV
LIVRVRHGHEDWGNEP

Nucleotide

• Length: 291 bp

>T158178 NZ_CP069594:c684936-684646 [Pseudomonas sp. Y39-6]
ATGAGCGAATCCTTTACCCGTTTCGATGCCGCAAAATACCTTAAGACTCCTTTTGAGATGGCCGCCTATCTGGATGCTTG
CTTTGAAGAAGATGCTGGCGACGGTCTGTTGATTCGCACAGCGTTGAACGATATCGCTCGCGCCCAAGGCATGACCCAAG
TCGCTCGCGACGCCGGCCTAGGACGAGAAAGTTTGTACAAAGCCCTCTCCAGCACTGGCAATCCCGAGTTCGCGACTATC
ATGAAGGTCATGAGAGCACTGGGGCTCAAACTTCACGCCACCGCGCTCTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9300.50 Da
• Isoelectric Point: 7.0134

>AT158178 WP_107341193.1 NZ_CP053856:444049-444300 [Rhizobium pusense]
MVTMNISLPGQMKEWVEAQSKTGRYSNASDYVRDLIRKDQERSEKIAAMQGLVDEGLQAPSGHRSEDALFAEAEARSMKR
KIG

Nucleotide

• Length: 252 bp

>AT158178 NZ_CP069594:c685242-684940 [Pseudomonas sp. Y39-6]
ATGATTGAGTTCGAAGAATCCGTTGAGTTTGTCAAATGGCTCGAAGCAATGCGCGATCCGCTCGCAAAAGTACGCATTGT
GACAAGGCTGCGCATGGCGCAAGCAGGAAACTTCGGCGACTGTGAGGCTGTAGGAGAGGGCGTTTGCGAAATGCGAATCC
ACCAAGGCCCCGGGTATCGGGTTTACTTCACTCTCCGAGACAAGGTGGTTTATCTGCTGCTGATGGGCGGCGACAAATCA
ACACAAAGCCGAGACATCCAGCGTGCAATACACATGGCAAAAAATCTTGGCCGCAAGGAGTGA

Structures

Toxin

• AlphaFold DB: U4PRC3