Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2636642..2637267 Replicon chromosome
Accession NZ_CP053855
Organism Escherichia coli strain 258E

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag HPY99_RS12520 Protein ID WP_000911330.1
Coordinates 2636869..2637267 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag HPY99_RS12515 Protein ID WP_000450524.1
Coordinates 2636642..2636869 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY99_RS12490 (2632445) 2632445..2632915 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
HPY99_RS12495 (2632915) 2632915..2633487 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
HPY99_RS12500 (2633633) 2633633..2634511 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
HPY99_RS12505 (2634528) 2634528..2635562 + 1035 WP_001330579.1 outer membrane protein assembly factor BamC -
HPY99_RS12510 (2635775) 2635775..2636488 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
HPY99_RS12515 (2636642) 2636642..2636869 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
HPY99_RS12520 (2636869) 2636869..2637267 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
HPY99_RS12525 (2637414) 2637414..2638277 + 864 WP_001267498.1 neutral zinc metallopeptidase -
HPY99_RS12530 (2638292) 2638292..2640307 + 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
HPY99_RS12535 (2640381) 2640381..2641079 + 699 WP_000679823.1 esterase -
HPY99_RS12540 (2641189) 2641189..2641389 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T158167 WP_000911330.1 NZ_CP053855:2636869-2637267 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T158167 NZ_CP069589:4124600-4124887 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT158167 WP_000450524.1 NZ_CP053855:2636642-2636869 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT158167 NZ_CP069589:4124874-4125176 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACACCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCACTGGCGAA
TAAATTCGACGAAGATATTGATTACAGTGATATCCCGCCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACCCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGCTGAAGCGACCTGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References