Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 1625082..1625713 Replicon chromosome
Accession NZ_CP053855
Organism Escherichia coli strain 258E

Toxin (Protein)


Gene name hicA Uniprot ID F4NNK6
Locus tag HPY99_RS07545 Protein ID WP_001259388.1
Coordinates 1625082..1625357 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag HPY99_RS07550 Protein ID WP_000593555.1
Coordinates 1625354..1625713 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY99_RS07530 (1620085) 1620085..1621153 + 1069 Protein_1463 HlyD family secretion protein -
HPY99_RS07535 (1621150) 1621150..1623885 + 2736 WP_000149125.1 ribosome-associated ATPase/putative transporter RbbA -
HPY99_RS07540 (1623885) 1623885..1625009 + 1125 WP_001314210.1 ABC transporter permease -
HPY99_RS07545 (1625082) 1625082..1625357 + 276 WP_001259388.1 type II toxin-antitoxin system HicA family toxin Toxin
HPY99_RS07550 (1625354) 1625354..1625713 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
HPY99_RS07555 (1625833) 1625833..1626234 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
HPY99_RS07560 (1626240) 1626240..1627046 - 807 WP_124783472.1 nickel import ATP-binding protein NikE -
HPY99_RS07565 (1627043) 1627043..1627807 - 765 WP_001136236.1 nickel import ATP-binding protein NikD -
HPY99_RS07570 (1627807) 1627807..1628640 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
HPY99_RS07575 (1628637) 1628637..1629581 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10151.89 Da        Isoelectric Point: 10.7076

>T158163 WP_001259388.1 NZ_CP053855:1625082-1625357 [Escherichia coli]
MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T158163 NZ_CP069589:3841504-3841677 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTTTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT158163 WP_000593555.1 NZ_CP053855:1625354-1625713 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT158163 NZ_CP069589:3841180-3841476 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A0FFA2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References