Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 877609..878106 Replicon chromosome
Accession NZ_CP053855
Organism Escherichia coli strain 258E

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HPY99_RS04145 Protein ID WP_001173343.1
Coordinates 877609..877782 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HPY99_RS04150 Protein ID WP_000398619.1
Coordinates 877810..878106 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPY99_RS04135 (874279) 874279..875736 + 1458 WP_000856834.1 dipeptide/tripeptide permease DtpC -
HPY99_RS04140 (875973) 875973..877490 + 1518 WP_001295087.1 lysine--tRNA ligase -
HPY99_RS04145 (877609) 877609..877782 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HPY99_RS04150 (877810) 877810..878106 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HPY99_RS04155 (878333) 878333..878605 - 273 WP_000405649.1 GNAT family N-acetyltransferase -
HPY99_RS04160 (878617) 878617..878847 - 231 WP_000371700.1 4Fe-4S mono-cluster protein YjdI -
HPY99_RS04165 (879028) 879028..880659 + 1632 WP_001216487.1 sensor histidine kinase -
HPY99_RS04170 (880656) 880656..881375 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T158161 WP_001173343.1 NZ_CP053855:c877782-877609 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T158161 NZ_CP069589:c3130758-3130651 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT158161 WP_000398619.1 NZ_CP053855:c878106-877810 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT158161 NZ_CP069589:3130807-3130872 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References