Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 4920399..4921015 Replicon chromosome
Accession NZ_CP053852
Organism Escherichia coli strain CE1628

Toxin (Protein)


Gene name vapC Uniprot ID A0A176ZIX8
Locus tag HPM96_RS23540 Protein ID WP_001129490.1
Coordinates 4920641..4921015 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1M0CY48
Locus tag HPM96_RS23535 Protein ID WP_001275523.1
Coordinates 4920399..4920641 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM96_RS23515 4915634..4916071 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
HPM96_RS23520 4916116..4917057 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
HPM96_RS23525 4917473..4919287 + 1815 WP_000226324.1 hypothetical protein -
HPM96_RS23530 4919961..4920179 + 219 WP_001251290.1 ribbon-helix-helix domain-containing protein -
HPM96_RS23535 4920399..4920641 + 243 WP_001275523.1 ribbon-helix-helix domain-containing protein Antitoxin
HPM96_RS23540 4920641..4921015 + 375 WP_001129490.1 type II toxin-antitoxin system VapC family toxin Toxin
HPM96_RS23545 4921052..4921981 - 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
HPM96_RS23550 4921978..4922613 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
HPM96_RS23555 4922610..4923512 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-115)

Antitoxin

Ribbon-helix-helix protein, CopG

(15-48)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13960.19 Da        Isoelectric Point: 7.0126

>T158157 WP_001129490.1 NZ_CP053852:4920641-4921015 [Escherichia coli]
MAKRTALFDTNILIDLFSGRIEAKQALEAWPLQNAISLVTWMEVLVGAKKYDQEHRTRIAMSAFNVIGISQDIAERSVAL
RQEYRMKLPDAIILATAQIYGFELVTRNTRDFAGIAGVVTPYEL

Download         Length: 375 bp

>T158157 NZ_CP069589:c2675018-2674704 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9290.54 Da        Isoelectric Point: 4.3774

>AT158157 WP_001275523.1 NZ_CP053852:4920399-4920641 [Escherichia coli]
MSAMAGMDMGRILLDLSDEVIKRLDDLKVQRNLPRAELLREAVDQYLENQSKTTISSAFGIWQGCEEDGVEYQRKLREEW

Download         Length: 243 bp

>AT158157 NZ_CP069589:c2674707-2674291 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A176ZIX8

Antitoxin

Source ID Structure
AlphaFold DB A0A1M0CY48

References