Detailed information of TA system TA158153

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3843509..3844307
• Replicon: chromosome
• Accession: NZ_CP053852
• Organism: Escherichia coli strain CE1628

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: U9XMP3
• Locus tag: HPM96_RS18490
• Protein ID: WP_000854735.1
• Coordinates: 3843930..3844307 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A1M1EW42
• Locus tag: HPM96_RS18485
• Protein ID: WP_032153712.1
• Coordinates: 3843509..3843883 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPM96_RS18450	3839242..3840126	+	885	WP_024166630.1	50S ribosome-binding GTPase	-
HPM96_RS18455	3840245..3840922	+	678	WP_023155728.1	hypothetical protein	-
HPM96_RS18460	3840952..3841161	+	210	WP_023155727.1	DUF905 family protein	-
HPM96_RS18465	3841252..3842070	+	819	WP_001234693.1	DUF945 domain-containing protein	-
HPM96_RS18470	3842162..3842647	+	486	WP_032153711.1	antirestriction protein	-
HPM96_RS18475	3842663..3843139	+	477	WP_001186715.1	RadC family protein	-
HPM96_RS18480	3843208..3843429	+	222	WP_000692345.1	DUF987 domain-containing protein	-
HPM96_RS18485	3843509..3843883	+	375	WP_032153712.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
HPM96_RS18490	3843930..3844307	+	378	WP_000854735.1	TA system toxin CbtA family protein	Toxin
HPM96_RS18495	3844304..3844795	+	492	WP_023155722.1	hypothetical protein	-
HPM96_RS18500	3844807..3845004	+	198	WP_000839265.1	DUF957 domain-containing protein	-
HPM96_RS18505	3845089..3845955	+	867	WP_032153714.1	DUF4942 domain-containing protein	-
HPM96_RS18510	3846027..3846290	+	264	WP_025492088.1	type II toxin-antitoxin system ParD family antitoxin	-
HPM96_RS18515	3846287..3846613	+	327	WP_000779483.1	type II toxin-antitoxin system RelE/ParE family toxin	-
HPM96_RS18520	3847089..3847259	+	171	Protein_3625	IS110 family transposase	-
HPM96_RS18525	3848070..3849053	+	984	WP_001361242.1	KpsF/GutQ family sugar-phosphate isomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3847155..3847259	104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14061.07 Da
• Isoelectric Point: 8.2904

>T158153 WP_000854735.1 NZ_CP053852:3843930-3844307 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T158153 NZ_CP069589:c1383902-1383651 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 13697.51 Da
• Isoelectric Point: 6.6240

>AT158153 WP_032153712.1 NZ_CP053852:3843509-3843883 [Escherichia coli]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYMAVYPTLAPATTS

Nucleotide

• Length: 375 bp

>AT158153 NZ_CP069589:c1383654-1383400 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: U9XMP3

Antitoxin

• AlphaFold DB: A0A1M1EW42