Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3698061..3698715 Replicon chromosome
Accession NZ_CP053852
Organism Escherichia coli strain CE1628

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag HPM96_RS17765 Protein ID WP_000244781.1
Coordinates 3698061..3698468 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag HPM96_RS17770 Protein ID WP_000354046.1
Coordinates 3698449..3698715 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM96_RS17745 3694018..3695751 - 1734 WP_000813184.1 single-stranded-DNA-specific exonuclease RecJ -
HPM96_RS17750 3695757..3696467 - 711 WP_000715220.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
HPM96_RS17755 3696492..3697388 - 897 WP_000806650.1 site-specific tyrosine recombinase XerD -
HPM96_RS17760 3697500..3698021 + 522 WP_001055874.1 flavodoxin FldB -
HPM96_RS17765 3698061..3698468 - 408 WP_000244781.1 protein YgfX Toxin
HPM96_RS17770 3698449..3698715 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
HPM96_RS17775 3698958..3699938 + 981 WP_000886072.1 tRNA-modifying protein YgfZ -
HPM96_RS17780 3700015..3700674 - 660 WP_000250274.1 hemolysin III family protein -
HPM96_RS17785 3700838..3701149 - 312 WP_001182965.1 N(4)-acetylcytidine aminohydrolase -
HPM96_RS17790 3701194..3702627 + 1434 WP_001361333.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T158152 WP_000244781.1 NZ_CP053852:c3698468-3698061 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T158152 NZ_CP069589:c1383654-1383400 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT158152 WP_000354046.1 NZ_CP053852:c3698715-3698449 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT158152 NZ_CP069589:c1383902-1383651 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGACAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References