Detailed information of TA system TA158140

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 1378527..1379232
• Replicon: chromosome
• Accession: NZ_CP053852
• Organism: Escherichia coli strain CE1628

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A1M2J6W7
• Locus tag: HPM96_RS06770
• Protein ID: WP_000539524.1
• Coordinates: 1378846..1379232 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: HPM96_RS06765
• Protein ID: WP_001280945.1
• Coordinates: 1378527..1378856 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPM96_RS06750	1373685..1374596	+	912	WP_001236044.1	glutathione ABC transporter permease GsiD	-
HPM96_RS06755	1374773..1377121	+	2349	WP_000950331.1	EAL domain-containing protein	-
HPM96_RS06760	1377129..1378457	+	1329	WP_000086870.1	GGDEF domain-containing protein	-
HPM96_RS06765	1378527..1378856	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
HPM96_RS06770	1378846..1379232	-	387	WP_000539524.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
HPM96_RS06775	1379458..1380783	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
HPM96_RS06780	1380996..1381379	+	384	WP_000497137.1	biofilm formation regulator BssR	-
HPM96_RS06785	1381490..1382605	+	1116	WP_000555056.1	aldose sugar dehydrogenase YliI	-
HPM96_RS06790	1382602..1383228	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14321.46 Da
• Isoelectric Point: 9.9521

>T158140 WP_000539524.1 NZ_CP053852:c1379232-1378846 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSRSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T158140 NZ_CP069583:c4436057-4435707 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT158140 WP_001280945.1 NZ_CP053852:c1378856-1378527 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT158140 NZ_CP069583:c4436302-4436051 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A1M2J6W7