Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 950600..951218 Replicon chromosome
Accession NZ_CP053852
Organism Escherichia coli strain CE1628

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag HPM96_RS04635 Protein ID WP_001291435.1
Coordinates 950600..950818 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag HPM96_RS04640 Protein ID WP_000344800.1
Coordinates 950844..951218 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM96_RS04600 945888..946460 + 573 WP_000779831.1 YbaY family lipoprotein -
HPM96_RS04605 946491..946832 - 342 WP_151394824.1 MGMT family protein -
HPM96_RS04615 947182..947535 + 354 WP_000878140.1 DUF1428 domain-containing protein -
HPM96_RS04620 947577..949127 - 1551 WP_001361689.1 cyclic-guanylate-specific phosphodiesterase PdeB -
HPM96_RS04625 949291..949761 - 471 WP_000136192.1 YlaC family protein -
HPM96_RS04630 949877..950428 - 552 WP_000102574.1 maltose O-acetyltransferase -
HPM96_RS04635 950600..950818 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
HPM96_RS04640 950844..951218 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
HPM96_RS04645 951764..954913 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
HPM96_RS04650 954936..956129 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T158138 WP_001291435.1 NZ_CP053852:c950818-950600 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T158138 NZ_CP069583:4303447-4303704 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT158138 WP_000344800.1 NZ_CP053852:c951218-950844 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT158138 NZ_CP069583:4303716-4304267 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References