Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 368339..368915 Replicon chromosome
Accession NZ_CP053852
Organism Escherichia coli strain CE1628

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag HPM96_RS01760 Protein ID WP_001131965.1
Coordinates 368339..368626 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2U2VE39
Locus tag HPM96_RS01765 Protein ID WP_000063158.1
Coordinates 368613..368915 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM96_RS01730 363748..365160 - 1413 WP_000199309.1 PLP-dependent aminotransferase family protein -
HPM96_RS01735 365337..365501 + 165 WP_000394263.1 DUF1127 domain-containing protein -
HPM96_RS01740 365543..365881 - 339 WP_000132633.1 endoribonuclease SymE -
HPM96_RS01745 366096..366764 - 669 Protein_343 DUF3387 domain-containing protein -
HPM96_RS01750 366758..366931 - 174 Protein_344 SAM-dependent DNA methyltransferase -
HPM96_RS01755 366976..368142 - 1167 WP_000800823.1 restriction endonuclease -
HPM96_RS01760 368339..368626 + 288 WP_001131965.1 BrnT family toxin Toxin
HPM96_RS01765 368613..368915 + 303 WP_000063158.1 BrnA antitoxin family protein Antitoxin
HPM96_RS01770 368949..369905 - 957 WP_001361361.1 GTPase -
HPM96_RS01775 369916..370119 - 204 WP_000467859.1 YbdD/YjiX family protein -
HPM96_RS01780 370169..372319 - 2151 WP_001314407.1 pyruvate/proton symporter BtsT -
HPM96_RS01785 372587..373807 - 1221 WP_180815801.1 amino acid deaminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T158136 WP_001131965.1 NZ_CP053852:368339-368626 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T158136 NZ_CP069583:c4143531-4143247 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11537.99 Da        Isoelectric Point: 9.4646

>AT158136 WP_000063158.1 NZ_CP053852:368613-368915 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDSDVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT158136 NZ_CP069583:c4143767-4143534 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2U2VE39

References