Detailed information of TA system TA158134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 262102..262697
• Replicon: chromosome
• Accession: NZ_CP053852
• Organism: Escherichia coli strain CE1628

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: HPM96_RS01280
• Protein ID: WP_000239581.1
• Coordinates: 262347..262697 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: HPM96_RS01275
• Protein ID: WP_001223213.1
• Coordinates: 262102..262353 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
HPM96_RS01265	257767..261546	+	3780	WP_000060873.1	autotransporter assembly complex protein TamB	-
HPM96_RS01270	261549..261890	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
HPM96_RS01275	262102..262353	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
HPM96_RS01280	262347..262697	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
HPM96_RS01285	262776..263306	-	531	WP_000055072.1	inorganic diphosphatase	-
HPM96_RS01290	263616..264572	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
HPM96_RS01295	264705..266207	+	1503	WP_001095661.1	sugar ABC transporter ATP-binding protein	-
HPM96_RS01300	266221..267243	+	1023	WP_001361374.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T158134 WP_000239581.1 NZ_CP053852:262347-262697 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T158134 NZ_CP069583:3691969-3692187 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT158134 WP_001223213.1 NZ_CP053852:262102-262353 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT158134 NZ_CP069583:3691569-3691943 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7