Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 154796..155293 Replicon chromosome
Accession NZ_CP053852
Organism Escherichia coli strain CE1628

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag HPM96_RS00735 Protein ID WP_001173343.1
Coordinates 155120..155293 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag HPM96_RS00730 Protein ID WP_000398619.1
Coordinates 154796..155092 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HPM96_RS00710 151525..152244 - 720 WP_000611288.1 two-component system response regulator DcuR -
HPM96_RS00715 152241..153872 - 1632 WP_061092758.1 sensor histidine kinase -
HPM96_RS00720 154053..154283 + 231 WP_000371704.1 (4Fe-4S)-binding protein -
HPM96_RS00725 154295..154567 + 273 WP_000405647.1 N-acetyltransferase -
HPM96_RS00730 154796..155092 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
HPM96_RS00735 155120..155293 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
HPM96_RS00740 155412..156929 - 1518 WP_000003806.1 lysine--tRNA ligase -
HPM96_RS00745 157166..158623 - 1458 WP_061092757.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T158133 WP_001173343.1 NZ_CP053852:155120-155293 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T158133 NZ_CP069583:2811423-2811530 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT158133 WP_000398619.1 NZ_CP053852:154796-155092 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT158133 NZ_CP069583:c2811366-2811311 [Escherichia coli]
CAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References